Assessment of ileal pouch inflammation by single-stool calprotectin assay
| Autor: | Ingvar Bjarnason, Ashley B. Price, Paul Thomas, Hanan Rihani, G Sigthorsson, R. John Nicholls, Arne Røseth |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Pathology medicine.medical_specialty Neutrophils Biopsy T-Lymphocytes Enzyme-Linked Immunosorbent Assay Pouchitis Familial adenomatous polyposis Diagnosis Differential Feces fluids and secretions medicine Humans Neural Cell Adhesion Molecules Lamina propria Gastrointestinal tract Membrane Glycoproteins medicine.diagnostic_test business.industry Macrophages Calcium-Binding Proteins Gastroenterology Colonoscopy General Medicine medicine.disease Ulcerative colitis medicine.anatomical_structure Female Calprotectin Pouch business Leukocyte L1 Antigen Complex Biomarkers |
| Zdroj: | Diseases of the Colon & Rectum. 43:214-220 |
| ISSN: | 0012-3706 |
| DOI: | 10.1007/bf02236986 |
| Popis: | PURPOSE: Assessment of inflammation within the ileal pouch to establish a diagnosis of “pouchitis” requires both pouch endoscopy and biopsy because there can be a poor correlation between macroscopic and histologic assessments of inflammation. A simplified diagnostic test would be of clinical advantage. Calprotectin is a stable myelomonocytic protein, measurable in feces. It quantitatively relates to inflammation within the gastrointestinal tract. This study was designed to compare single and 24-hour stool measurements of calprotectin in patients with and without evidence of ileal pouch inflammation with endoscopic, histologic, and immunohistochemical indices. METHODS: Twenty-four-hour stool collections were made in ileal pouch patients, 9 with and 15 without (7 with ulcerative colitis and 8 with familial polyposis coli) evidence of pouch inflammation. First-morning stool concentration and total 24-hour calprotectin were quantified by use of a single step enzyme-linked immunosorbent assay. Biopsies from the reservoir were taken for conventional histology and scoring of intraepithelial neutrophil infiltrate. Cells positive for CD3, CD45RO, CD14, and CD15 within the lamina propria were quantified by use of immunohistochemistry. RESULTS: The mean first-morning stool calprotectin concentration correlated with the 24-hour level (r=0.91;P= |
| Databáze: | OpenAIRE |
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