Transcriptome-Based Analysis Reveals Therapeutic Effects of Resveratrol on Endometriosis in aRat Model
Autor: | Chunyan Wang, Xiaohang Yang, Xianlei Zhao, Cuicui Lin, Xiaomeng Shi, Min-Xin Guan, Zhengyun Chen, Mengdan Zhao, Yuhan Lou, Shenghui Hong, Yongmei Xi |
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Rok vydání: | 2021 |
Předmět: |
endometriosis
PPARγ glucose tolerance Endometriosis Anti-Inflammatory Agents Pharmaceutical Science Peroxisome proliferator-activated receptor Pharmacology Resveratrol resveratrol Lesion Rats Sprague-Dawley chemistry.chemical_compound Insulin resistance Adipocyte Drug Discovery Medicine Animals Protein kinase B Original Research chemistry.chemical_classification Drug Design Development and Therapy business.industry food and beverages Lipid metabolism medicine.disease Lipid Metabolism Rats Disease Models Animal chemistry Female medicine.symptom Insulin Resistance RNA-seq business Transcriptome Signal Transduction |
Zdroj: | Drug Design, Development and Therapy |
ISSN: | 1177-8881 |
Popis: | Chunyan Wang,1,2,* Zhengyun Chen,1,* Xianlei Zhao,2,* Cuicui Lin,2 Shenghui Hong,3 Yuhan Lou,2 Xiaomeng Shi,1 Mengdan Zhao,1 Xiaohang Yang,1,2 Min-Xin Guan,2 Yongmei Xi1,2 1The Womenâs Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, Peopleâs Republic of China; 2Institute of Genetics, Zhejiang University; Department of Human Genetics, Zhejiang University School of Medicine, Zhejiang Provincial Key Laboratory of Genetic & Developmental Disorders, Hangzhou, Zhejiang, 310058, Peopleâs Republic of China; 3Laboratory Animal Center of Zhejiang University, Hangzhou, Zhejiang, 310001, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Yongmei Xi; Min-Xin Guan Tel +86-0571-88206623; +86-0571-88981371Email xyyongm@zju.edu.cn; gminxin88@zju.edu.cnIntroduction: Endometriosis (EMs) is associated with severe chronic pelvic pain and infertility and the development of improved EMs treatment options is an ongoing focus. In this study, we investigated the effects of resveratrol on EMs and analyzed transcriptional changes in the lesions of model rats before and after resveratrol treatment.Methods: We established arat model of endometriosis through the trans-implantation of endometrial fragments to the peritoneal wall and then used resveratrol as treatment. We then analyzed the results using RNA sequencing of the lesion tissues of each of the model rats before resveratrol treatment and the reduced lesion tissues after the treatment. Examinations of anatomy, biochemistry, immunohistochemical staining and flow cytometry examinations were also conducted. Other trans-implanted rats were also given sham treatments as sham-treatment control and other untrans-implanted rats served as sham-operation controls.Results: In addition to the obvious lesions observed in the model rats, there were significant differences in the glucose tolerance, macrophage M1/M2 polarization, and adipocyte sizes between the treated model rats and sham (control) rats. Resveratrol treatment in the model rats showed significant efficacy and positive therapeutic effect. Transcriptional analysis showed that the effects of resveratrol on the endometriosis model rats were manifested by alterations in the PPAR, insulin resistance, MAPK and PI3K/Akt signaling pathways. Correspondingly, changes in PPARγ activation, M1/M2 polarization and lipid metabolism were also detected after resveratrol treatment.Discussion: Our study revealed that resveratrol treatment displayed efficient therapeutic effects for EMs model rats, probably through its important roles in anti-inflammation, immunoregulation and lipid-related metabolism regulation.Keywords: endometriosis, resveratrol, RNA-seq, glucose tolerance, PPARγ |
Databáze: | OpenAIRE |
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