Effect on sphingomyelin-containing liposomes of phospholipase D from Corynebacterium ovis and the cytolysin from Stoichactis helianthus
| Autor: | Alan W. Bernheimer, Regina Linder |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Biophysics
Corynebacterium medicine.disease_cause Biochemistry Hemolysin Proteins Endocrinology medicine Helianthus Glycoproteins Liposome biology Phospholipase D Toxin Vesicle food and beverages medicine.disease biology.organism_classification Hemolysis Sphingomyelins Sea Anemones Sphingomyelin Phosphodiesterase Phospholipases Liposomes Marine Toxins Cytolysin Sphingomyelin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 530:236-246 |
| ISSN: | 0005-2760 |
| DOI: | 10.1016/0005-2760(78)90009-7 |
| Popis: | The toxic, sphingomyelin-specific phospholipase D (phosphatidylcholine phosphatidohydrolase EC 3.1.4.4) from Corynebacterium ovis was purified to near homogeneity. It had a molecular weight of 31 000 and a pI of approx. 9.8. Although not cytolytic itself, it protected red cells from hemolysis by staphylococcal sphingomyelinase (β-hemolysin) and helianthus toxin. The apparently non-enzymatic cytolysin (helianthus toxin) from the sea anemone Stoichactis helianthus also interacts with membrane sphingomyelin. C. ovis and helianthus toxins were compared with regard to their effects on liposome model membranes, and they were found both to produce changes analogous to those in erythrocytes. Only helianthus toxin caused release of trapped glucose marker, but liposomes could be protected from release by pretreatment with C. ovis toxin. Both toxins demonstrated binding to sphingomyelin-containing liposomes, but only the bacterial sphingomyelinase catalyzed the release of choline from these vesicles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|