Infection stage-dependent modulation of macrophage activation in Trypanosoma congolense-resistant and -susceptible mice
| Autor: | Geert Raes, Boniface Namangala, Gholamreza Hassanzadeh, Patrick De Baetselier, Alain Beschin, Inge Daems, Frank Brombacher, Wim Noël, Lea Brys |
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| Přispěvatelé: | Cellular and Molecular Immunology, Chemical Engineering and Industrial Chemistry, Vrije Universiteit Brussel |
| Rok vydání: | 2002 |
| Předmět: |
Chemokine
Cellular immunity Trypanosoma congolense medicine.medical_treatment Immunology Parasitemia Microbiology Mice Immune system Nerve Growth Factor medicine Macrophage Animals Interleukin 4 Chemokine CCL2 Tumor necrosis factor secretion Mice Inbred BALB C Interleukin-13 biology Proteins Macrophage Activation Mice Inbred C57BL Infectious Diseases Cytokine Trypanosomiasis African Interleukin 13 biology.protein Cytokines Intercellular Signaling Peptides and Proteins Parasitology Disease Susceptibility Interleukin-4 Fungal and Parasitic Infections |
| Zdroj: | Vrije Universiteit Brussel |
| ISSN: | 0019-9567 |
| Popis: | The contribution of cytokines and chemokines to resistance and susceptibility to African trypanosomiasis remains controversial. In the present study, the levels of type I and type II cytokines and of the MCP-1 chemokine were compared during the early and late stages ofTrypanosoma congolenseinfection in susceptible BALB/c and resistant C57BL/6 mice. Moreover, the status of macrophage activation was compared in these animals by analyzing the inducible nitric oxide synthase-arginase balance, tumor necrosis factor secretion, and expression of the FIZZ1 and YM genes. Data show that changing from a predominant type I cytokine environment in the early stage of infection to a predominant type II cytokine environment and an enhanced MCP-1 secretion in the late stage of infection correlates with resistance toT. congolense. Concomitantly, macrophage activation evolves from a classical to a predominant alternative phenotype. We further confirmed that the simultaneous occurrence of type I/type II cytokines in the early stage of infection in susceptible BALB/c mice, reflected by the presence of macrophages exhibiting a mixed classical/alternative activation phenotype, is associated with uncontrolled parasite growth and early death. Interleukin-4 (IL-4) and IL-13 signaling did not influence the susceptibility of BALB/c mice toT. congolenseinfection and interestingly were not the main trigger to alternative macrophage activation. InT. congolense-resistant C57BL/6 mice, our results corroborated the induction of FIZZ1 and YM gene expressions with the alternative pathway of macrophage activation. In susceptible BALB/c mice, however, YM but not FIZZ1 induction reflected the emergence of alternatively activated macrophages. Hence, the FIZZ1 and YM genes may be useful markers to discriminate between distinct populations of alternatively activated macrophages. |
| Databáze: | OpenAIRE |
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