Cold Shock as a Screen for Genes Involved in Cold Acclimatization in Neurospora crassa
| Autor: | Nicholas Seitz, Nichole Walters, Michael K. Watters, Holly Howell, Alexander Mehreteab, Sienna Kekelik, Erik Rose, Jacob Nava, Victor Manzanilla, Laura Knuth, Brianna Scivinsky |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hyphal growth 030106 microbiology Mutant Screen Report Biology QH426-470 biology.organism_classification Neurospora Cold shock response Cell biology Neurospora crassa 03 medical and health sciences 030104 developmental biology cold shock cold adaptation morphology branching Genetics Transcription Factor Gene Molecular Biology Gene Transcription factor Genetics (clinical) Gene knockout |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 8, Iss 5, Pp 1439-1454 (2018) |
| ISSN: | 2160-1836 |
| Popis: | When subjected to rapid drops of temperature (cold shock), Neurospora responds with a temporary shift in its morphology. This report is the first to examine this response genetically. We report here the results of a screen of selected mutants from the Neurospora knockout library for alterations in their morphological response to cold shock. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. A total of 344 knockout strains were subjected to cold shock. Of those, 118 strains were identified with altered responses. We report here the cold shock morphologies and GO categorizations of strains subjected to this screen. Of strains with knockouts in genes associated with hyphal growth or morphology, 33 of 131 tested (25%) showed an altered response to cold shock. Of strains with knockouts in transcription factor genes, 30 of 145 (20%) showed an altered response to cold shock. Of strains with knockouts in genes homologous to E. coli genes which display altered levels of transcription in response to cold shock, a total of 55 of 68 tested (81%) showed an altered cold shock response. This suggests that the response to cold shock in these two organisms is largely shared in common. |
| Databáze: | OpenAIRE |
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