| Autor: |
Minhee Jung, Eunji Shin, Young-Hoon Kim, Daesik Kim, Joonsun Lee, Kang-In Lee, Euihwan Jeong, Seokjoong Kim, Jungjoon K Lee, Jin-Soo Kim |
| Rok vydání: |
2017 |
| Předmět: |
|
| DOI: |
10.1101/237040 |
| Popis: |
The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we have used a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specificities without sacrificing on-target activities in human cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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