Complete genome characterization of a rotavirus B (RVB) strain identified in Alpine goat kids with enteritis reveals inter-species transmission with RVB bovine strains
| Autor: | Fangzhou Chen, Douglas Marthaler, Max Ciarlet, Matthew Sturos, Todd P. Knutson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rotavirus Genotype viruses Short Communication 030106 microbiology Reassortment Cattle Diseases interspecies transmission Genome Viral Biology Genome Rotavirus Infections Enteritis 03 medical and health sciences Viral Proteins Phylogenetics Virology medicine Animals Gene Phylogeny NSP1 Goat Diseases Transmission (medicine) Animal Strain (biology) Goats virus diseases goat rotavirus B medicine.disease Double-strand RNA Viruses phylogenetics 030104 developmental biology Animals Newborn Cattle |
| Zdroj: | The Journal of General Virology |
| ISSN: | 1465-2099 |
| Popis: | Rotavirus B (RVB) has been associated with enteric disease in many animal species. An RVB strain was identified in pooled intestinal samples from Alpine caprine kids (between 2 and 3 days of age) experiencing high (>90 %) morbidity, and the complete caprine RVB genome was characterized. Histology revealed villus atrophy, the samples tested positive for RVB by real-time RT-PCR and metagenomic next-generation sequencing identified only RVB and orf virus. In the VP4 gene segment, the caprine RVB strain had a higher percentage nucleotide identity to the Indian bovine RVB strains than to the Japanese bovine RVB strains, but the VP7, VP6, VP2, NSP1, NSP2 and NSP5 gene segments of the American caprine RVB strain were genetically related to the Japanese bovine RVB strains. The results indicate a lack of RVB sequences to understand reassortment or the evolutionary relationship of RVB strains from cattle and goats. |
| Databáze: | OpenAIRE |
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