Nuclear import of activated D-ERK by DIM-7, an importin family member encoded by the gene moleskin
| Autor: | Danny L. Brower, James A. Lorenzen, Scott E. Baker, Fabienne Denhez, Lizabeth A. Perkins, Michael B. Melnick |
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| Rok vydání: | 2001 |
| Předmět: |
Molecular Sequence Data
Mutant Active Transport Cell Nucleus Importin Karyopherins medicine.disease_cause Receptor tyrosine kinase Cell Line Gene expression medicine Animals Drosophila Proteins Amino Acid Sequence Phosphorylation Molecular Biology Cell Nucleus Mutation Sequence Homology Amino Acid biology Schneider 2 cells Nuclear Proteins Protein Tyrosine Phosphatases Non-Receptor Molecular biology Enzyme Activation Drosophila melanogaster Mutagenesis biology.protein Tyrosine Mitogen-Activated Protein Kinases Protein Tyrosine Phosphatases Nuclear transport Nuclear localization sequence Subcellular Fractions Developmental Biology |
| Zdroj: | Development. 128:1403-1414 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | The initiation of gene expression in response to Drosophila receptor tyrosine kinase signaling requires the nuclear import of the MAP kinase, D-ERK. However, the molecular details of D-ERK translocation are largely unknown. In this regard, we have identified D-Importin-7 (DIM-7), the Drosophila homolog of vertebrate importin 7, and its gene moleskin. DIM-7 exhibits a dynamic nuclear localization pattern that overlaps the spatial and temporal profile of nuclear, activated D-ERK. Co-immunoprecipitation experiments show that DIM-7 associates with phosphorylated D-ERK in Drosophila S2 cells. Furthermore, moleskin mutations enhance hypomorphic and suppress hypermorphic D-ERK mutant phenotypes. Deletion or mutation of moleskin dramatically reduces the nuclear localization of activated D-ERK. Directly linking DIM-7 to its nuclear import, this defect can be rescued by the expression of wild-type DIM-7. Mutations in the Drosophila Importin β homolog Ketel, also reduce the nuclear localization of activated D-ERK. Together, these data indicate that DIM-7 and Ketel are components of the nuclear import machinery for activated D-ERK. |
| Databáze: | OpenAIRE |
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