Impact of p16INK4a Immunohistochemistry Staining on Interobserver Agreement on the Diagnosis of Cervical Intraepithelial Neoplasia
| Autor: | Ilaria Sabatucci, Roberto Pisa, Vincenzo Maccallini, Paolo Giorgi Rossi, B. Ghiringhello, Alberto Pacchiarotti, Simona Galeotti, Giovanni Negri, Fabio Musolino, Paola Bellardini, Guido Collina, Paolo Palma, Francesco Chini |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
medicine.medical_specialty Population Uterine Cervical Neoplasms Cervical intraepithelial neoplasia Biopsy Biomarkers Tumor medicine Humans education Cyclin-Dependent Kinase Inhibitor p16 Mass screening Observer Variation Gynecology Colposcopy Cervical cancer education.field_of_study medicine.diagnostic_test business.industry Reproducibility of Results General Medicine Middle Aged Uterine Cervical Dysplasia medicine.disease Immunohistochemistry Confidence interval Staining Female Nuclear medicine business |
| Zdroj: | American Journal of Clinical Pathology. 141:367-373 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1309/ajcpcywvl61svkfu |
| Popis: | This study aimed to compare the interobserver Cohen κ on HE staining and on HE plus p16(INK4a) staining of all cervical biopsy specimens in a population-based screening program.All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with HE and HE plus p16. Biopsy specimens were reviewed by six external experts.The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with HE and HE + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively.The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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