Inhibition of NTPDase, 5′-nucleotidase, Na+/K+-ATPase and acetylcholinesterase activities by subchronic treatment with Casearia sylvestris
| Autor: | G.D. Zanetti, Daniela Balz, Maria Rosa Chitolina Schetinger, Maísa Corrêa, Vera Maria Morsch, A. C. F. da Silva, J.B.D’A. de Souza, M.P. Manfron |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Nucleotidase activity Aché Pharmaceutical Science Pharmacology 5'-nucleotidase chemistry.chemical_compound Antigens CD Casearia sylvestris Nucleotidase Drug Discovery Animals Enzyme Inhibitors Rats Wistar Na+/K+-ATPase 5'-Nucleotidase Cerebral Cortex Plant Extracts Chemistry Apyrase Cell Membrane Acetylcholinesterase language.human_language Rats Plant Leaves Complementary and alternative medicine Casearia language Molecular Medicine Cholinergic Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Phytomedicine. 13:509-514 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2005.01.011 |
| Popis: | The aqueous extract of Casearia sylvestris was tested in cortical membrane preparations. C. sylvestris was obtained commercially from two different sources, designated as Sample A and Sample B. The enzymes studied in this work were NTPDase-like, 5'-Nucleotidase, Na(+)/K(+)-ATPase and acetylcholinesterase (AChE). Adult rats received aqueous extracts from C. sylvestris in a dose of 20mg/kg body wt. daily for a 75-day-period, by oral administration (gavage). Our study showed that this treatment caused an inhibition of NTPDase-like activity with both, ATP (19.41% with Sample A and 25.03% with Sample B) and ADP (41.57% with Sample A and 31.20% with Sample B) as substrates. This treatment also caused an inhibition of 5'-nucleotidase activity (28.34% with Sample A and 31.46% with Sample B) and Na(+)/K(+)-ATPase (25.08% with Sample A and 24.81% with Sample B). The rate of acetylcholine degradation was reduced, as shown by the inhibition of AChE (31.65% and 26.74%, Samples A and B, respectively). These results suggest that extracts of C. sylvestris can cause neurochemical alterations in the purinergic and cholinergic systems of the central nervous system. |
| Databáze: | OpenAIRE |
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