Up-regulation of dopamine D2L mRNA levels in the ventral tegmental area and dorsal striatum of amphetamine-sensitized C57BL/6 mice: role of Cav1.3 L-type Ca2+channels
| Autor: | Anjali M. Rajadhyaksha, Joerg Striessnig, Shirish S. Satpute, Barry E. Kosofsky, T. P. Giordano |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Calcium Channels L-Type Amphetamine-Related Disorders Striatum Motor Activity Nucleus accumbens Biochemistry Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Dopamine Internal medicine Basal ganglia medicine Animals RNA Messenger Amphetamine Neurotransmitter Mice Knockout Voltage-dependent calcium channel Receptors Dopamine D2 Chemistry Ventral Tegmental Area Up-Regulation Mice Inbred C57BL Neostriatum Ventral tegmental area Disease Models Animal Endocrinology medicine.anatomical_structure Central Nervous System Stimulants medicine.drug |
| Zdroj: | Journal of Neurochemistry. 99:1197-1206 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.2006.04186.x |
| Popis: | Dopamine D(2) long (D(2)L) and D(2) short (D(2)S) isoforms of the D(2) receptor play an important role in psychostimulant-induced neuronal adaptations. In this study, we used quantitative real-time PCR to specifically amplify these two splice variants to examine their mRNA expression in the dorsal striatum (dStr), nucleus accumbens (NAc) and the ventral tegmental area (VTA) of amphetamine-sensitized C57BL/6 mice. We found a significant increase in D(2)L mRNA in the VTA and dStr of amphetamine-treated mice that positively correlated with the sensitized locomotor response. We also found a significant increase in D(2)S mRNA in the VTA. We further examined the role of the Ca(v)1.3 subtype of L-type Ca(2+) channels in up-regulation of D(2)L and D(2)S mRNA in the VTA. Amphetamine-pretreated Ca(v)1.3 wild-type (Ca(v)1.3(+/+)) mice exhibited sensitized behavior and a significant increase in D(2)L and D(2)S mRNA compared with saline-pretreated mice Amphetamine-pretreated homozygous Ca(v)1.3 knockout (Ca(v)1.3(-/-)) mice did not exhibit sensitized behavior. There was a significant increase in D(2)S mRNA, but not D(2)L mRNA. In conclusion, our results find that amphetamine increases D(2)L mRNA expression in the dStr and the VTA, an adaptation that correlates with expression of sensitized behavior and dependence on Ca(v)1.3 Ca(2+) channels. |
| Databáze: | OpenAIRE |
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