Distinct TFIID complexes mediate the effect of different transcriptional activators
| Autor: | Irwin Davidson, Jun Wu, Laszlo Tora, S Chaudhary, Yves Lutz, Christel Brou, Pierre Chambon, Jean-Marc Egly |
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| Přispěvatelé: | Laboratoire de Génetique Moléculaire des Eucaryotes (LGME), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Předmět: |
Transcription
Genetic [SDV]Life Sciences [q-bio] genetic processes MESH: TATA-Box Binding Protein Biological Factors Mice 0302 clinical medicine MESH: Animals 0303 health sciences biology General Neuroscience MESH: Transcription Factor TFIID MESH: Transcription Factors Cell biology DNA-Binding Proteins TAF1 030220 oncology & carcinogenesis TAF2 Female Transcription factor II A Research Article Recombinant Fusion Proteins TATA box information science macromolecular substances General Biochemistry Genetics and Molecular Biology 03 medical and health sciences MESH: Recombinant Fusion Proteins Animals Humans MESH: Mice Molecular Biology 030304 developmental biology TATA-Binding Protein Associated Factors MESH: Humans General Immunology and Microbiology MESH: Transcription Genetic TATA-Box Binding Protein Molecular biology MESH: HeLa Cells Transcription Factor TFIID MESH: Biological Factors health occupations biology.protein TATA-binding protein MESH: Chromatography Liquid MESH: Female MESH: DNA-Binding Proteins Chromatography Liquid HeLa Cells Transcription Factors |
| Zdroj: | Scopus-Elsevier Europe PubMed Central EMBO Journal EMBO Journal, EMBO Press, 1993, 12 (2), pp.489-99 |
| ISSN: | 0261-4189 1460-2075 |
| Popis: | International audience; Multiple chromatographically separable complexes containing the TATA binding protein (TBP), which exhibit different functional properties, exist in HeLa cells. At least three distinct subpopulations of such complexes can be functionally defined as TFIID since they function with RNA polymerase II. Using a partially reconstituted HeLa cell in vitro transcription system and immunoprecipitation with a monoclonal antibody directed against TBP, we show that stimulation of transcription by the chimeric activators GAL-VP16, GAL-TEF-1 and GAL-ER(EF) requires the presence of factors which are tightly associated with these TFIID complexes. Moreover, the activity of GAL-TEF-1 appears to be mediated by at least two chromatographically distinct populations of TFIID. The factor(s) associated with one of these populations is also required for the activity of GAL-ER (EF) and GAL-VP16, while the factor(s) associated with the other population functions selectively with GAL-TEF-1. These two TFIID populations are composed of both common and unique TBP associated factors (TAFs). |
| Databáze: | OpenAIRE |
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