Acetylcholine, GABA and glutamate induce ionic currents in cultured antennal lobe neurons of the honeybee, Apis mellifera
| Autor: | Guillaume Stephane Barbara, Jürgen Rybak, Monique Gauthier, Christina Zube, Bernd Grünewald |
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| Přispěvatelé: | Centre de Recherches sur la Cognition Animale (CRCA), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut fur Biologie, AG Neurobiologie, Freie Universität Berlin, Department of Behavioural Physiology and Sociobiology, Universitat Wurzburg Biozentrum, Massou, Isabelle |
| Předmět: |
Patch-Clamp Techniques
Epinephrine Physiology Models Neurological Glutamic Acid Nicotinic Antagonists Biology Inhibitory postsynaptic potential gamma-Aminobutyric acid Ion Channels Membrane Potentials GABA Antagonists 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine GABA receptor medicine Excitatory Amino Acid Agonists Animals Drug Interactions Neurons Afferent Nicotinic Agonists GABA Agonists Ecology Evolution Behavior and Systematics Cells Cultured gamma-Aminobutyric Acid 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug GABAA receptor Glutamate receptor Pupa Olfactory Pathways Bees Acetylcholine 3. Good health Nicotinic agonist medicine.anatomical_structure Biophysics Animal Science and Zoology Antennal lobe Neuroscience Excitatory Amino Acid Antagonists 030217 neurology & neurosurgery medicine.drug Ionotropic effect Histamine |
| Zdroj: | Scopus-Elsevier |
| Popis: | The honeybee, Apis mellifera, is a valuable model system for the study of olfactory coding and its learning and memory capabilities. In order to understand the synaptic organisation of olfactory information processing, the transmitter receptors of the antennal lobe need to be characterized. Using whole-cell patch-clamp recordings, we analysed the ligand-gated ionic currents of antennal lobe neurons in primary cell culture. Pressure applications of acetylcholine (ACh), gamma-amino butyric acid (GABA) or glutamate induced rapidly activating ionic currents. The ACh-induced current flows through a cation-selective ionotropic receptor with a nicotinic profile. The ACh-induced current is partially blocked by alpha-bungarotoxin. Epibatidine and imidacloprid are partial agonists. Our data indicate the existence of an ionotropic GABA receptor which is permeable to chloride ions and sensitive to picrotoxin (PTX) and the insecticide fipronil. We also identified the existence of a chloride current activated by pressure applications of glutamate. The glutamate-induced current is sensitive to PTX. Thus, within the honeybee antennal lobe, an excitatory cholinergic transmitter system and two inhibitory networks that use GABA or glutamate as their neurotransmitter were identified. |
| Databáze: | OpenAIRE |
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