High cell density cultivation of recombinant E. coli with a pressurized culture
| Autor: | Seigo Sato, Haruto Yokota, Naoya Shinzato, Toru Matsui, Joji Takahashi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_element
Bioengineering Tryptophan synthase Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Oxygen Microbiology law.invention chemistry.chemical_compound law medicine Food science Escherichia coli business.industry Cell growth High cell General Medicine Biotechnology chemistry Carbon dioxide Recombinant DNA biology.protein Specific activity business |
| Zdroj: | Process Biochemistry. 41:920-924 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2005.10.004 |
| Popis: | An air-pressurized culture was used to enhance the oxygen transfer rate in the high cell density cultivation (HCDC) of non-recombinant and recombinant (harbors pBR322trpAB) Escherichia coli K-12. Carbon dioxide generated by the cells under increased pressure was inhibitory and as a result, cellular growth stopped in the air-pressurized culture at a constant mass flow rate. Increasing the flow rate along with the pressure in the reactor enabled the cells to grow to 130 (non-recombinant) and 104 (recombinant) g/l due to the release of the CO 2 . In addition, the specific activity of tryptophan synthase was increased. This study showed that an air-pressurized culture is able to meet the high demand for oxygen in the HCDC of E. coli . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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