Hepatitis B surface antigen fusions delivered by DNA vaccination elicit CTL responses to human papillomavirus oncoproteins associated with tumor protection
| Autor: | Robert W. Tindle, Oscar Haigh, Jacqueline Kattenbelt, Melanie Cochrane, Allan R. Gould, Scott Thomson |
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| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
HBsAg Papillomavirus E7 Proteins Genetic Vectors Epitopes T-Lymphocyte Uterine Cervical Neoplasms Epitope DNA vaccination Mice Papillomavirus Vaccines Antigen Vaccines DNA Animals Medicine Cytotoxic T cell Molecular Biology Hepatitis B Surface Antigens business.industry Papillomavirus Infections virus diseases Oncogene Proteins Viral Virology Repressor Proteins Disease Models Animal CTL Immunology Carcinoma Squamous Cell Molecular Medicine Female business T-Lymphocytes Cytotoxic |
| Zdroj: | Cancer Gene Therapy. 17:708-720 |
| ISSN: | 1476-5500 0929-1903 |
| DOI: | 10.1038/cgt.2010.27 |
| Popis: | We describe the construction and evaluation of a recombinant hepatitis B surface antigen (HBsAg)-vectored DNA vaccine encoding the E7 and E6 tumor-associated oncoproteins of human papillomavirus (HPV) type 16. We show the induction of effector and memory cytotoxic T lymphocyte responses to E7 and E6 class I-restricted epitopes after a single immunization, which were associated with tumor prevention and therapy. The findings vindicate the use of a HBsAg-based DNA vaccine as a vehicle to elicit responses to co-encoded tumor antigens, and have specific implications for the development of a therapeutic vaccine for HPV-associated squamous carcinomas. |
| Databáze: | OpenAIRE |
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