Expression of clara cell secretory protein in the lungs of rats exposed to crystalline silica in vivo
| Autor: | Takako Oyabu, Yasuko Obata, Masami Hirohashi, Akira Ogami, Kaori Kuroda, Isamu Tanaka, Hiroshi Yamato, Toshiaki Higashi, Hiroko Nagatomo, Yasuo Morimoto |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Lung injury Andrology Western blot Japan In vivo medicine Animals Uteroglobin Secretion RNA Messenger Rats Wistar Saline Inhalation exposure Messenger RNA Inhalation Exposure Lung medicine.diagnostic_test Chemistry Reverse Transcriptase Polymerase Chain Reaction Public Health Environmental and Occupational Health Pneumonia respiratory system Silicon Dioxide Rats medicine.anatomical_structure |
| Zdroj: | Journal of occupational health. 47(6) |
| ISSN: | 1341-9145 |
| Popis: | It has been theorized that Clara cell secretion protein (CCSP) plays a critical role in regulating the acute inflammatory response in the lung. We hypothesized that CCSP is also related to lung injury induced by occupational dust. The present study was conducted to investigate the time course of the expression of CCSP in lungs exposed to crystalline silica in vivo. Male Wistar rats were administered 1 mg or 2 mg of silica suspended in saline by a single intratracheal instillation and were sacrificed at 3 d, 1 wk, 1 month, 3 months and 6 months of recovery time. The expression of CCSP was observed by RT-PCR and western blot analysis. Exposure to 2 mg of silica decreased in levels of CCSP mRNA at 3 d, 1 wk, 1 month and 6 months following intratracheal instillation. The protein level of CCSP in silica-exposed rats was decreased at 3 d, 7 d and 1 month after a single instillation of 2 mg. The decreases in CCSP at the acute phase in this experiment suggest that CCSP may regulate the acute injury of the lung exposed to silica. |
| Databáze: | OpenAIRE |
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