Acceleration effect of the forensic luminol reaction induced by visible light irradiation of whole human blood aqueous solutions
| Autor: | Nobuhiro Yukawa, Saaya Hayashi, Norihiro Shinkawa, Ai Sonoda, Tsutomu Shiragami, Eiji Kakizaki |
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| Rok vydání: | 2019 |
| Předmět: |
Light
Photochemistry 01 natural sciences Methemoglobin Pathology and Forensic Medicine Luminol law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Humans 030216 legal & forensic medicine Irradiation Sodium Azide Chemiluminescence Luminescent Agents Singlet oxygen 010401 analytical chemistry Forensic Medicine 0104 chemical sciences Oxygen Blood chemistry Luminescent Measurements Sodium azide Hemoglobin Law Visible spectrum |
| Zdroj: | Forensic Science International. 299:208-214 |
| ISSN: | 0379-0738 |
| DOI: | 10.1016/j.forsciint.2019.04.007 |
| Popis: | The first quantitative study on the effect of visible light irradiation on the luminol reaction, used forensically, was conducted using whole human blood aqueous solutions (hemolytic state) and an LED lamp. Whole human blood aqueous solutions under an air atmosphere were irradiated with visible light, resulting in the maximum chemiluminescence (CL) intensity (@ 440 nm) increasing about 1.7-fold due to acceleration of the luminol reaction rate. No acceleration effect was observed under an argon (Ar) atmosphere, or under an air atmosphere in the presence of sodium azide (NaN3; a scavenger of singlet oxygen (1O2)). Furthermore, no conversion from Fe(II) hemoglobin to Fe(III) hemoglobin (methemoglobin) was observed in the absorption spectrum following irradiation. We suggest that these effects are due to easier approach of the luminol reagents to heme following damage of the globin protein around the heme, or damage to the red blood cell membrane, induced by 1O2 generated by an excited state of heme. |
| Databáze: | OpenAIRE |
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