Excess Testosterone Exposure Alters Hypothalamic-Pituitary-Testicular Axis Dynamics and Gene Expression in Sheep Fetuses
| Autor: | Rebecka Amodei, Charles E. Roselli, Keely R. Corder, Fred Stormshak, Charles T. Estill, Kyle P. Gribbin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Testosterone propionate Hypothalamo-Hypophyseal System endocrine system medicine.medical_specialty Genotype medicine.drug_class Hypothalamus Biology Real-Time Polymerase Chain Reaction Gonadotropin-Releasing Hormone 03 medical and health sciences chemistry.chemical_compound Fetus Endocrinology Kisspeptin Pregnancy Internal medicine Testis medicine Animals Estrogen Receptor beta Testosterone RNA Messenger Maternal-Fetal Exchange Sheep Domestic Original Research Kisspeptins Leydig cell Androgen Gonadotropin secretion 030104 developmental biology medicine.anatomical_structure chemistry Maternal Exposure Estrogen Female Gonadotropin Gonadotropins hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 157:4234-4245 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2016-1411 |
| Popis: | Prenatal exposure to excess androgen may result in impaired adult fertility in a variety of mammalian species. However, little is known about what feedback mechanisms regulate gonadotropin secretion during early gestation and how they respond to excess T exposure. The objective of this study was to determine the effect of exogenous exposure to T on key genes that regulate gonadotropin and GnRH secretion in fetal male lambs as compared with female cohorts. We found that biweekly maternal testosterone propionate (100 mg) treatment administered from day 30 to day 58 of gestation acutely decreased (P < .05) serum LH concentrations and reduced the expression of gonadotropin subunit mRNA in both sexes and the levels of GnRH receptor mRNA in males. These results are consistent with enhanced negative feedback at the level of the pituitary and were accompanied by reduced mRNA levels for testicular steroidogenic enzymes, suggesting that Leydig cell function was also suppressed. The expression of kisspeptin 1 mRNA, a key regulator of GnRH neurons, was significantly greater (P < .01) in control females than in males and reduced (P < .001) in females by T exposure, indicating that hypothalamic regulation of gonadotropin secretion was also affected by androgen exposure. Although endocrine homeostasis was reestablished 2 weeks after maternal testosterone propionate treatment ceased, additional differences in the gene expression of GnRH, estrogen receptor-β, and kisspeptin receptor (G protein coupled receptor 54) emerged between the treatment cohorts. These changes suggest the normal trajectory of hypothalamic-pituitary axis development was disrupted, which may, in turn, contribute to negative effects on fertility later in life. |
| Databáze: | OpenAIRE |
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