Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer
| Autor: | Alison M. Rich, Lara T. Friedlander, Haizal Mohd Hussaini, V. Praveen Parachuru, Kullasit Chutipongpisit |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty government.form_of_government CD34 Vesicular Transport Proteins Fluorescent Antibody Technique Antigens CD34 Immunofluorescence Pathology and Forensic Medicine medicine Lymphatic vessel Biomarkers Tumor Humans Lymphangiogenesis Molecular Biology Lymphatic Vessels Homeodomain Proteins medicine.diagnostic_test business.industry Squamous Cell Carcinoma of Head and Neck Tumor Suppressor Proteins Endothelial Cells Cell Biology Original Articles Vascular Endothelial Growth Factor Receptor-3 Immunohistochemistry Endothelial stem cell stomatognathic diseases Lymphatic Endothelium medicine.anatomical_structure Lymphatic system government Mouth Neoplasms Endothelium Lymphatic business |
| Zdroj: | Int J Exp Pathol |
| Popis: | Lymphangiogenesis makes an important contribution to the tumour microenvironment (TME), but little is known about this in oral squamous cell carcinoma (OSCC). Archival formalin‐fixed paraffin‐embedded specimens (28 OSCC, 10 inflamed and 6 normal oral mucosa controls) were processed using immunohistochemistry (IHC) with antibodies against lymphatic markers D2‐40 (podoplanin), LYVE‐1, VEGFR3 and Prox1. After the endothelial cells had been highlighted by the various markers for lymphatic endothelium, the positive stained cells and vessels were identified and counted in a systematic manner to determine microvessel density. Double‐labelling immunofluorescence (DLIF) was used to investigate the specificity of D2‐40 and LYVE‐1 to lymphatic endothelial cells (LECs) as opposed to blood ECs. There was higher D2‐40 and Prox1 lymphatic vessel density (P = .001) in the OSCC group when compared with both control groups. Some malignant keratinocytes expressed lymphatic markers, as did a much smaller number of epithelial cells in the control groups. DLIF showed that no vessels co‐expressed D2‐40/CD34 or LYVE/CD34. Some D2/40(+) LVs were LYVE(−). D2‐40 was the most specific LEC marker in OSCC tissues. These results establish that the OSCC TME contains significantly more lymphatic vessels expressing D2‐40 and Prox1 than the control groups, which may play a role in facilitating lymphatic invasion and metastases. |
| Databáze: | OpenAIRE |
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