Compensatory Base Changes Reveal Sexual Incompatibility among Members of the Anopheles subpictus Sensu Lato (Diptera: Culicidae) Species Complex in Sri Lanka
| Autor: | Naduviladath Vishvanath Chandrasekharan, Dona P.W. Jayatunga, B.G.D.N.K. De Silva, I.N. Harischandra |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Species complex Anopheles subpictus health care facilities manpower and services 030231 tropical medicine General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine sibling species Sensu parasitic diseases medicine internal transcribed spacer 2 (ITS2) Internal transcribed spacer lcsh:Science Compensatory base changes (CBCs) health care economics and organizations Ecology Evolution Behavior and Systematics biology Anopheles Paleontology social sciences Reproductive isolation ITS2 secondary structures biology.organism_classification medicine.disease 030104 developmental biology Space and Planetary Science Evolutionary biology Vector (epidemiology) lcsh:Q geographic locations Malaria |
| Zdroj: | Life, Vol 11, Iss 211, p 211 (2021) Life Volume 11 Issue 3 |
| ISSN: | 2075-1729 |
| DOI: | 10.3390/life11030211 |
| Popis: | The mosquito Anopheles (Cellia) subpictus sensu lato (s.l.) is a major secondary vector of malaria in Sri Lanka. The sibling species composition in this species complex in Sri Lanka remains debatable. Compensatory base changes (CBCs) in the secondary structures of internal transcribed spacer 2 (ITS2) are reliable sources to predict sexual incompatibility among closely related species. The objective of the present study was to investigate the An. subpictus s.l. populations in Sri Lanka using the CBC analysis. Mosquito DNA was amplified and sequenced for the ITS2 region. The sequences were annotated using ITS2 Database. ITS2 secondary structures were constructed and analyzed for CBCs using various bioinformatics tools. The ITS2 regions consisted of two different lengths, 575 bp and 480 bp. The two CBCs and three hemi CBCs identified in the present study suggest that there may be at least two sexually incompatible sibling species. In conclusion, it is likely that there may be only two reproductively isolated sibling species in the An. subpictus species complex in Sri Lanka. However, due to high divergence of ITS2 in these species, it is reasonable to assume that they may be undergoing a speciation event to separate as a distinct species. |
| Databáze: | OpenAIRE |
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