Stability of the mercaptobenzothiazole compounds
| Autor: | Agrup G, Christer Hansson |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Antimetabolites
Morpholines Mercapto mix Sulfenamide Dermatology Glutathione Buffer solution Patch Tests Chemical reaction High-performance liquid chromatography chemistry.chemical_compound Thiazoles chemistry Drug Stability Immunology and Allergy Organic chemistry Mercaptobenzothiazole Benzothiazoles Sulfhydryl Compounds Pharmaceutical Vehicles Hapten Chromatography High Pressure Liquid |
| Zdroj: | Contact dermatitis. 28(1) |
| ISSN: | 0105-1873 |
| Popis: | An analytical quantitative high-pressure liquid chromatography (HPLC) method was developed for simultaneous determination of all mercaptobenzothiazole derivatives in the mercapto mix patch testing standard. The stability of the mercaptobenzothiazoles constituting the mercapto mix was studied both in petrolatum and in buffer solution at pH 6.5, with and without glutathione. In petrolatum vehicle, dibenzothiazyl disulfide was the dominant compound found in stored mercapto mix. In buffer solution at pH 6.5, 2-mercaptobenzothiazole and the sulfenamide derivatives morpholinyl mercaptobenzothiazole and N-cyclohexyl-2-benzothiazyl sulfenamide were converted into dibenzothiazyl disulfide. In the presence of glutathione, both the sulfenamide derivatives and the dibenzothiazyl disulfide were rapidly converted into 2-mercaptobenzothiazole. The findings explain the "cross-sensitivities" reported for the mercaptobenzothiazole group as a result of chemical reactions resulting in one main hapten. The use of a single substance for patch testing for mercaptobenzothiazole hypersensitivity is proposed. |
| Databáze: | OpenAIRE |
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