Antimicrobial resistance and genetic characterization of fluoroquinolone-resistant Mannheimia haemolytica isolates from cattle with bovine pneumonia
| Autor: | Mariko Kohmoto, Ken Katsuda, Ikuo Uchida, Osamu Mikami |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nalidixic acid
medicine.drug_class Molecular Sequence Data Colony Count Microbial Microbial Sensitivity Tests Drug resistance Biology Microbiology Antibiotic resistance Drug Resistance Multiple Bacterial Ampicillin Enrofloxacin medicine Animals Pasteurellosis Pneumonic Serotyping Mannheimia haemolytica Antiinfective agent General Veterinary Sequence Analysis DNA General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Antimicrobial Quinolone Virology Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field bacteria Cattle Fluoroquinolones medicine.drug |
| Zdroj: | Veterinary Microbiology. 139:74-79 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2009.04.020 |
| Popis: | Antimicrobial susceptibility and molecular characterization of quinolone-resistant Mannheimia haemolytica was conducted. The antimicrobial susceptibility of 229 M. haemolytica isolates which were obtained from cattle with bovine respiratory disease during the period 1984-2006, was determined using 14 antimicrobial agents. Of the 229 isolates, 114 (49.8%) were resistant to at least one agent and resistance rates ranged from 4.8% to 31.4%. Resistance rates for dihydrostreptomycin, oxytetracycline, doxycycline, ampicillin, amoxicillin, thiamphenicol, kanamycin chloramphenicol, nalidixic acid, enrofloxacin, and danofloxacin were 31.4%, 20.5%, 18.3%, 19.2%, 16.6%, 10.9%, 11.4%, 10.5%, 17.0%, 4.8% and 4.8%, respectively. The nucleotide sequences of the quinolone resistance-determining regions of the gyrA and parC genes of nalidixic acid-resistant M. haemolytica were determined. All nalidixic acid-resistant strains possessed at least one amino acid substitution in each of the GyrA and ParC fragments investigated. These results suggest that M. haemolytica require at least one amino acid substitution in both GyrA and ParC in order to attain significant levels of resistance to quinolones. All fluoroquinolone-resistant isolates belonged to serotype 6, and their genotype by PFGE analysis was identical. This result indicates that fluoroquinolone-resistant M. haemolytica strains have clonally expanded. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect