Prevalence of glutathione S-transferase gene deletions and their effect on sickle cell patients
| Autor: | Mishra Rahasya Mani, Pandey Sanjay, Ahuja Rajesh Kumar, Shah Vineet, Pandey Sweta, Saxena Renu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Pathology Anemia Thalassemia polymerase chain reaction Gastroenterology sickle cell Hemoglobin A2 Internal medicine Genotype glutathione transferase medicine hemoglobinopathies biology business.industry lcsh:RC633-647.5 Hematology lcsh:Diseases of the blood and blood-forming organs medicine.disease anemia Sickle cell anemia Hemoglobin A Glutathione S-transferase Hemoglobin F biology.protein Original Article business |
| Zdroj: | Revista Brasileira de Hematologia e Hemoterapia, Vol 34, Iss 2, Pp 100-102 (2012) Revista Brasileira de Hematologia e Hemoterapia, Volume: 34, Issue: 2, Pages: 100-102, Published: 2012 Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia v.34 n.2 2012 Revista brasileira de hematologia e hemoterapia Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC |
| ISSN: | 1806-0870 1516-8484 |
| Popis: | BACKGROUND: Glutathione S-transferase gene deletions are known detoxification agents and cause oxidative damage. Due to the different pathophysiology of anemia in thalassemia and sickle cell disease, there are significant differences in the pathophysiology of iron overload and iron-related complications in these disorders. OBJECTIVE: The aim of this study was to estimate the frequency of the GSTM1 and GSTT1 genotypes in sickle cell disease patients and their effect on iron status. METHODS: Forty sickle cell anemia and sixty sickle ß-thalassemia patients and 100 controls were evaluated to determine the frequency of GST gene deletions. Complete blood counts were performed by an automated cell analyzer. Hemoglobin F, hemoglobin A, hemoglobin A2 and hemoglobin S were measured and diagnosis of patients was achieved by high performance liquid chromatography with DNA extraction by the phenol-chloroform method. The GST null genotype was determined using multiplex polymerase chain reaction and serum ferritin was measured using an ELISA kit. Statistical analysis was by EpiInfo and GraphPad statistics software. RESULTS: An increased frequency of the GSTT1 null genotype (p-value = 0.05) was seen in the patients. The mean serum ferritin level was higher in patients with the GST genotypes than in controls; this was statistically significant for all genotypes except GSTM1, however the higher levels of serum ferritin were due to blood transfusions in patients. CONCLUSION: GST deletions do not play a direct role in iron overload of sickle cell patients. |
| Databáze: | OpenAIRE |
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