Role of high expression levels of STK39 in the growth, migration and invasion of non-small cell type lung cancer cells
| Autor: | Xi Chen, Qiang Lin, Zhao Li, Xiaobo Yu, Liwen Xiong, Wenzhuo Zhu |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Lung Neoplasms Carcinogenesis Apoptosis Kaplan-Meier Estimate NSCLC medicine.disease_cause Metastasis 0302 clinical medicine Cell Movement Carcinoma Non-Small-Cell Lung RNA Small Interfering Aged 80 and over STK39 Cell Cycle High-Throughput Nucleotide Sequencing Middle Aged Cell cycle Flow Cytometry Prognosis Up-Regulation Oncology Lymphatic Metastasis 030220 oncology & carcinogenesis Female RNA Interference Research Paper Adult medicine.medical_specialty MAP Kinase Signaling System proliferation Down-Regulation Protein Serine-Threonine Kinases Malignancy 03 medical and health sciences Cell Line Tumor Biomarkers Tumor medicine Humans metastasis RNA Messenger Lung cancer neoplasms Aged Cell Proliferation Neoplasm Staging Oncogene Sequence Analysis RNA business.industry Cell growth Gene Expression Profiling Cancer Oncogenes medicine.disease respiratory tract diseases 030104 developmental biology Cancer research business |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Zhao Li 1, * , Wenzhuo Zhu 1, * , Liwen Xiong 2 , Xiaobo Yu 1 , Xi Chen 1 , Qiang Lin 1 1 Department of Thoracic Surgery, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China 2 Department of Pulmonary Diseases, Shanghai Chest Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China * These authors contributed equally to this work Correspondence to: Qiang Lin, email: xklinqiang@hotmail.com Keywords: STK39, NSCLC, proliferation, metastasis Received: June 07, 2016 Accepted: July 30, 2016 Published: August 17, 2016 ABSTRACT Non-small cell type lung cancer (NSCLC) is the most common malignancy and the leading cause of cancer related mortality. In this study, serine/threonine kinase 39 (STK39) was identified as an up-regulated gene in NSCLC tissues by next-generation RNA sequencing. Although STK39 gene polymorphisms may be prognostic of overall survival in patients with early stage NSCLC, the roles of STK39 in NSCLC cancer are poorly understood. In the current study, Genome Set Enrichment Analysis (GSEA) on the RNA-seq data of NSCLC specimens indicated that cancer-related process and pathways, including metastasis, cell cycle, apoptosis and p38 pathway, were significantly correlated with STK39 expression. STK39 expression was significantly increased in NSCLC cases and its protein expression was positively correlated with the poor tumor stage, large tumor size, advanced lymphnode metastasis and poor prognosis. Down-regulation of STK39 in NSCLC cells significantly decreased cell proliferation by blocking of cell cycle and inducing apoptosis. We also found that STK39 knockdown in NSCLC cells remarkably repressed cell migration and invasion. On the contrary, overexpression of STK39 in NSCLC cells had inverse effects on cell behaviors. Taken together, STK39 acts as a tumor oncogene in NSCLC and can be a potential biomarker of carcinogenesis. |
| Databáze: | OpenAIRE |
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