The second messenger cyclic di-AMP negatively regulates the expression of Mycobacterium smegmatis recA and attenuates DNA strand exchange through binding to the C-terminal motif of mycobacterial RecA proteins
| Autor: | Sadaf Dabeer, Deepika Prasad, Ankita Srivastava, Kalappa Muniyappa, Anuja Krishnan, Kasi Manikandan, Nirpendra Singh, Krishna M. Sinha |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA repair 030106 microbiology Mutant Mycobacterium smegmatis DNA Single-Stranded medicine.disease_cause Microbiology Second Messenger Systems Biochemistry 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins medicine Cyclic AMP Escherichia coli Molecular Biology Gene Cyclic GMP biology Recombinational DNA Repair Gene Expression Regulation Bacterial Mycobacterium tuberculosis biology.organism_classification Cell biology DNA-Binding Proteins Rec A Recombinases Nucleoproteins chemistry Second messenger system bacteria Homologous recombination DNA |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | Cyclic di-GMP and cyclic di-AMP are second messengers produced by a wide variety of bacteria. They influence bacterial cell survival, biofilm formation, virulence and bacteria-host interactions. However, many of their cellular targets and biological effects are yet to be determined. A chemical proteomics approach revealed that Mycobacterium smegmatis RecA (MsRecA) possesses a high-affinity cyclic di-AMP binding activity. We further demonstrate that both cyclic di-AMP and cyclic di-GMP bind specifically to the C-terminal motif of MsRecA and Mycobacterium tuberculosis RecA (MtRecA). Escherichia coli RecA (EcRecA) was devoid of cyclic di-AMP binding but have cyclic di-GMP binding activity. Notably, cyclic di-AMP attenuates the DNA strand exchange promoted by MsRecA as well as MtRecA through the disassembly of RecA nucleoprotein filaments. However, the structure and DNA strand exchange activity of EcRecA nucleoprotein filaments remain largely unaffected. Furthermore, M. smegmatis disA cells were found to have undetectable RecA levels due to the translational repression of recA mRNA. Consequently, the Delta disA mutant exhibited enhanced sensitivity to DNA-damaging agents. Altogether, this study points out the importance of sequence diversity among recA genes, the role(s) of cyclic di-AMP and reveals a new mode of negative regulation of recA gene expression, DNA repair and homologous recombination in mycobacteria. |
| Databáze: | OpenAIRE |
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