CF1-dependent restoration of energy-linked reactions reconstituted with a hydrophobic protein from spinach chloroplasts
| Autor: | Hassan M. Younis, G. Douglas Winget |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Halobacterium
Ammonium sulfate Chloroplasts Proteolipids Biophysics Fractionation Biochemistry Catalysis chemistry.chemical_compound Adenosine Triphosphate Molecular Biology Phospholipids Plant Proteins Adenosine Triphosphatases biology Vesicle Extraction (chemistry) Bacteriorhodopsin Cell Biology Plants Chloroplast Kinetics Membrane Oxidative Phosphorylation Coupling Factors chemistry Bacteriorhodopsins biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 77:168-174 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(77)80179-4 |
| Popis: | Summary The hydrophobic membrane component of chloroplast ATPase (F o ) was purified by extraction of CF 1 -depleted chloroplasts with cholate and fractionation of the extract with ammonium sulfate. Vesicles, reconstituted with F o and phospholipids, catalyzed 32p i -ATP exchange when supplemented with CF 1 . When bacteriorhodopsin was incorporated into these vesicles light-dependent ATP formation was catalyzed. |
| Databáze: | OpenAIRE |
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