Comparison of Replica Leaf Surface Materials for Phyllosphere Microbiology
| Autor: | Rebecca Soffe, Volker Nock, Michal Bernach, Mitja N. P. Remus-Emsermann, Nicola Altenhuber |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Leaves Atmospheric Science bepress|Engineering Swine Plant Science medicine.disease_cause Gelatin Pattern Recognition Automated chemistry.chemical_compound Animal Products Materials Physics Image Processing Computer-Assisted Surface Energy Microstructure Wax Microscopy Multidisciplinary Plant Anatomy Physics food and beverages Agriculture Condensed Matter Physics Atomic Force Microscopy engrXiv|Engineering visual_art engrXiv|Engineering|Materials Science and Engineering|Biology and Biomimetic Materials Physical Sciences visual_art.visual_art_medium Agarose Medicine bepress|Engineering|Materials Science and Engineering engrXiv|Engineering|Materials Science and Engineering Research Article food.ingredient Materials science animal structures Surface Properties Science 030106 microbiology Materials Science Research and Analysis Methods Microbiology 03 medical and health sciences bepress|Engineering|Materials Science and Engineering|Biology and Biomimetic Materials food Meteorology Mold medicine Animals Microbial Viability Polydimethylsiloxane Bacteria Pantoea Replica Scanning Probe Microscopy fungi Organisms Biology and Life Sciences Humidity Surface energy Plant Leaves 030104 developmental biology chemistry Earth Sciences Phyllosphere |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 6, p e0218102 (2019) |
| DOI: | 10.31224/osf.io/2pzrv |
| Popis: | Artificial surfaces are routinely used instead of leaves to enable a reductionist approach in phyllosphere microbiology, the study of microorganisms residing on plant leaf surfaces. Commonly used artificial surfaces include, flat surfaces, such as metal and nutrient agar, and microstructured surfaces, such as isolate leaf cuticles or reconstituted leaf waxes. However, interest in replica leaf surfaces as an artificial surface is growing, as replica surfaces provide an improved representation of the complex topography of leaf surfaces. To date, leaf surfaces have predominantly been replicated for their superhydrophobic properties. In contrast, in this paper we investigated the potential of agarose, the elastomer polydimethylsiloxane (PDMS), and gelatin as replica leaf surface materials for phyllosphere microbiology studies. Using a test pattern of pillars, we investigated the ability to replicate microstructures into the materials, as well as the degradation characteristics of the materials in environmental conditions. Pillars produced in PDMS were measured to be within 10% of the mold master and remained stable throughout the degradation experiments. In agarose and gelatin the pillars deviated by more than 10% and degraded considerably within 48 hours in environmental conditions. Furthermore, we investigated the surface energy of the materials, an important property of a leaf surface, which influences resource availability and microorganism attachment. We found that the surface energy and bacterial viability on PDMS was comparable to isolated Citrus × aurantium and Populus × canescens leaf cuticles. Hence indicating that PDMS is the most suitable material for replica leaf surfaces. In summary, our experiments highlight the importance of considering the inherent material properties when selecting a replica leaf surface for phyllosphere microbiology studies. As demonstrated, a PDMS replica leaf offers a control surface that can be used for investigating microbe-microbe and microbe-plant interactions in the phyllosphere, which will enable mitigation strategies against pathogens to be developed. |
| Databáze: | OpenAIRE |
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