Independent inhibition of DNA synthesis by protein kinase C, cyclic AMP and interferon α/β in rabbit aortic smooth muscle cells
| Autor: | Yoshimi Takai, Hisashi Fukuzaki, Yasuo Fukumoto, Yasuhiro Kawahara, Ken-ichi Kariya, Shun-ichi Araki |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Inositol Phosphates Biophysics Alpha interferon Phospholipase Biology Biochemistry Muscle Smooth Vascular Interferon Internal medicine Cyclic AMP medicine Animals Alprostadil Beta (finance) Protein kinase A Molecular Biology Cells Cultured Protein Kinase C Protein kinase C DNA synthesis Cell Cycle DNA Cell Biology Molecular biology Endocrinology Cell culture Interferon Type I Tetradecanoylphorbol Acetate Rabbits medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 157:337-345 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(88)80052-4 |
| Popis: | In quiescent cultures of rabbit aortic smooth muscle cells, whole blood serum-induced DNA synthesis was inhibited markedly by protein kinase C-activating 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), cyclic AMP-derivatives, such as dibutyryl cyclic AMP (Bt2cAMP) and 8-bromo-cyclic AMP, and interferon alpha/beta. Neither TPA nor interferon alpha/beta elevated the cellular cyclic AMP level. Neither Bt2cAMP nor interferon alpha/beta induced the phospholipase C-mediated hydrolysis of phosphoinositides. The down-regulation of protein kinase C by prolonged treatment with PDBu abolished the antiproliferative action of TPA but did not affect that of Bt2cAMP or interferon alpha/beta. TPA and Bt2cAMP inhibited the serum-induced DNA synthesis when added within 12 h after the addition of the serum, while interferon alpha/beta was active only when added within 6 h. These results suggest that there are at least three independent signaling systems, protein kinase C- and cyclic AMP-mediated systems and an unidentified system for interferon alpha/beta, which are involved in the antiproliferative mechanisms in rabbit aortic smooth muscle cells. |
| Databáze: | OpenAIRE |
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