Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip
| Autor: | Sabine Lutz-Bonengel, Timo Sänger, Ulrike Schön, Ulrike Schmidt, Marielle Heinrich |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Mitochondrial DNA
Multiple displacement amplification Biology Chip DNA Fingerprinting DNA Mitochondrial Polymerase Chain Reaction Molecular biology Pathology and Forensic Medicine Nuclear DNA Electrophoresis Microchip Low volume chemistry.chemical_compound chemistry Humans Microsatellite Amelogenin DNA Microsatellite Repeats Oligonucleotide Array Sequence Analysis |
| Zdroj: | International Journal of Legal Medicine. 121:68-73 |
| ISSN: | 1437-1596 0937-9827 |
| Popis: | Low volume (LV) amplification (1 microL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure--except for the purification of the sequencing products--were accomplished within the same final 2-microL reaction volume. |
| Databáze: | OpenAIRE |
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