Measurement of mRNA of 11 biomarkers by RT-PCR to detect lymph node involvement in cervical cancer
Autor: | Françoise Révillion, N Alloy, Jean-Philippe Peyrat, Valérie Lhotellier, Eric Leblanc, Vanessa Samouëlian |
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Rok vydání: | 2008 |
Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Cancer Research Pathology medicine.medical_specialty Receptor ErbB-4 Clinical Biochemistry Uterine Cervical Neoplasms Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Biomarkers Tumor medicine Humans RNA Messenger Lymph node MUC1 Keratin-19 Cervical cancer Reverse Transcriptase Polymerase Chain Reaction Mucin-1 Cancer medicine.disease Immunohistochemistry Urokinase-Type Plasminogen Activator ErbB Receptors Reverse transcription polymerase chain reaction 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure Matrix Metalloproteinase 9 Oncology 030220 oncology & carcinogenesis Lymphatic Metastasis Female Lymph |
Zdroj: | The International Journal of Biological Markers. 23:74-82 |
ISSN: | 0393-6155 |
Popis: | Lymph node metastases are a major prognostic factor in cervical carcinomas. The aim of this study was to characterize the expression of 11 markers in cervical tumors and negative lymph nodes and to determine which ones could be helpful for improving the specificity of molecular diagnosis of nodal involvement. Using TaqMan RT-PCR, we studied the expression of CK19, MUC1, HER1–HER4, VEGF, VEGF-C, uPA, MMP9, and PRAD1 in uterine cervical tumors and in histologically nonmetastatic lymph nodes of 8 patients diagnosed with locally advanced cervical cancer. We observed that CK19, MUC1, HER1–HER3, uPA, and VEGF had a significantly higher expression in cervical tumors than in the negative nodes, whereas VEGF-C expression level was higher in the negative nodes than in the tumors. PRAD1 harbored similar expression levels in the tumors and in the negative nodes. Interestingly, 1 of the 4 patients who presented a clinical recurrence, showed elevated HER1, HER2, uPA, and VEGF in the histologically negative nodes. Our results suggest that CK19, MUC1, HER1–3, uPA, and VEGF are biomarkers that have a higher expression in tumoral cervical tissues compared with the negative lymph nodes and could be useful to diagnose nodal involvement in uterine cervical carcinoma. Our results should encourage us in continue to investigate a greater number of patients, including patients with histologically involved nodes. |
Databáze: | OpenAIRE |
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