A Comparison of Vascularity, Bone Mineral Density Distribution, and Histomorphometrics in an Isogenic Versus an Outbred Murine Model of Mandibular Distraction Osteogenesis
| Autor: | Noah S. Nelson, Peter A. Felice, Stephen Y. Kang, Sagar S. Deshpande, Catherine N. Tchanque-Fossuo, Steven R. Buchman, Edward G. Carey, Alexis Donneys, Alexander R. Zheutlin, Kathleen K. Gallagher |
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| Rok vydání: | 2016 |
| Předmět: |
Male
X-ray microtomography Bone density medicine.medical_treatment Osteogenesis Distraction Mandible Rats Sprague-Dawley 03 medical and health sciences External fixation Mice 0302 clinical medicine Vascularity Bone Density medicine Animals 030223 otorhinolaryngology Bone mineral business.industry Anatomy X-Ray Microtomography Osteotomy Sprague dawley Disease Models Animal Otorhinolaryngology Density distribution Mandibular distraction Rats Inbred Lew 030220 oncology & carcinogenesis Surgery Oral Surgery medicine.symptom business |
| Zdroj: | Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons. 74(10) |
| ISSN: | 1531-5053 |
| Popis: | Purpose The vascularity, bone mineral density distribution, and histomorphometric data between the inbred, isogenic Lewis rat and the outbred, nonisogenic Sprague Dawley rat within mandibular distraction osteogenesis (MDO) were evaluated to allow future researchers to compare the results generated from these 2 animals. We hypothesized that little difference would be found between the 2 strains within these metrics. Materials and Methods We implemented a comparative study between the Lewis and Sprague Dawley rat strains within MDO. The sample was composed of 17 male Lewis and 17 male Sprague Dawley rats that underwent surgical external fixation and distraction. The rats' hemimandibles were distracted to a total distance of 5.1 mm. After 28 days of consolidation, 9 rats from each group underwent bone mineral density distribution analysis. The remaining rats from each group were analyzed for the vascular and histologic metrics. Descriptive and bivariate statistics were computed, and the P value was set at .05. Results We demonstrated successful MDO in all the rats, with no significant difference found in the histologic or bone mineral density distribution metrics. No significant differences were found in any of the vascular metrics, with the exception of vascular separation, which was not normalized to the mandibular volume (P = .048). Conclusions The results of the present study have demonstrated that little dissimilarity exists between the isogenic Lewis and outbred Sprague Dawley models of MDO. Thus, researchers can confidently compare the gross results between the 2 strains, with consideration of the very small differences between the 2 models. For studies that require an isogenic strain, the Lewis rat is an apt surrogate for the Sprague Dawley strain. |
| Databáze: | OpenAIRE |
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