Quantitative flow cytometry shows activation of the TNF-alpha system but not of the IL-2 system at the single cell level in renal replacement therapy
| Autor: | Robert Zietse, Willem Weimar, Iza C. van Riemsdijk‐van Overbeeke, C. J. Knoop, E.H.M. Loonen, Carla C. Baan |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Interleukin 2
Adult Male medicine.medical_treatment T-Lymphocytes Lymphocyte Activation Monocytes Receptors Tumor Necrosis Factor Flow cytometry chemistry.chemical_compound Immune system Peritoneal Dialysis Continuous Ambulatory Antigens CD Reference Values Renal Dialysis medicine Humans Protein Isoforms Receptors Tumor Necrosis Factor Type II IL-2 receptor Fluorescein isothiocyanate Receptor Netherlands Transplantation medicine.diagnostic_test business.industry Racial Groups Interleukin Receptors Interleukin-2 Middle Aged Flow Cytometry Cytokine chemistry Nephrology Immunology Calibration Kidney Failure Chronic Female business Biomarkers medicine.drug |
| Zdroj: | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 16(7) |
| ISSN: | 0931-0509 |
| Popis: | textabstractBACKGROUND: Immunological dysfunction in patients on haemodialysis may be related to imbalanced cytokine systems, such as tumour necrosis factor (TNF)-alpha and interleukin (IL)-2. Despite activation of these systems, haemodialysis patients show high susceptibility for infections and malignancies, and have a poor immunological reaction to T-cell-dependent antigens, like hepatitis B vaccination. In this study we have determined the activation status of the two different cytokine systems, at the single cell level, using quantitative flow cytometry. METHODS: Using fluorescein isothiocyanate- or phycoerythrin-conjugated antibodies directed against TNF-R2 (CD120b), IL-2Ralpha (CD25) and IL-2Rbeta (CD122), we measured the expression of these receptors at the single cell level in order to determine the level of activation of monocytes and T-lymphocytes. RESULTS: Significantly higher expression of the TNF-alpha receptor, TNF-R2, was present on both monocytes and T-lymphocytes in patients on renal replacement therapy (RRT) compared with pre-dialysis chronic renal failure (CRF) patients and controls, indicating activation of the TNF-alpha system. In contrast, IL-2R expression was comparable in all groups studied, which may reflect a non-activated state of the IL-2 system. CONCLUSIONS: The present study illustrates an activated state of the TNF-alpha system in patients on RRT, at the single cell level, while the IL-2 system seems to be unaffected. These findings support the hypothesis that the interaction between the TNF-alpha and IL-2 cytokine systems is disturbed. |
| Databáze: | OpenAIRE |
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