An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury
| Autor: | Jun Li, Feng Feng, Liming Li, Liumin He, Jinghua Luo, Zan Tan, Xiaoying Wang, Xueshuang Shi, Mao Pang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Curcumin
Neurite QH301-705.5 0206 medical engineering Biomedical Engineering Schwann cell 02 engineering and technology Spinal cord injury Article Biomaterials Extracellular matrix medicine Schwann cells Remyelination Biology (General) Materials of engineering and construction. Mechanics of materials Chemistry technology industry and agriculture Injectable and self-healing hydrogel 021001 nanoscience & nanotechnology medicine.disease Spinal cord 020601 biomedical engineering Controlled release medicine.anatomical_structure Self-healing hydrogels Biophysics TA401-492 0210 nano-technology Biotechnology |
| Zdroj: | Bioactive Materials Bioactive Materials, Vol 6, Iss 12, Pp 4816-4829 (2021) |
| ISSN: | 2452-199X |
| Popis: | The harsh local micro-environment following spinal cord injury (SCI) remains a great challenge for neural regeneration. Local reconstitution of a favorable micro-environment by biocompatible scaffolds with desirable functions has thus been an area of concern. Herein, a hybrid hydrogel was developed using Fmoc-grafted chitosan (FC) and Fmoc peptide (FI). Dynamic reversible π-π stacking interactions of the fluorenyl rings enabled the FC/FI hybrid hydrogel to exhibit excellent injectable and self-healing properties, as characterized by visual appearances and rheological tests. Furthermore, the FC/FI hybrid hydrogel showed a slow and persistent release of curcumin (Cur), which was named as FC/FI-Cur hydrogel. In vitro studies confirmed that with the support of FC/FI-Cur hydrogel, neurite outgrowth was promoted, and Schwann cell (SC) migration away from dorsal root ganglia (DRG) spheres with enhanced myelination was substantiated. The FC/FI-Cur hydrogel well reassembled extracellular matrix at the lesion site of rat spinal cord and exerted outstanding effects in modulating local inflammatory reaction by regulating the phenotypes of infiltrated inflammatory cells. In addition, endogenous SCs were recruited in the FC/FI-Cur graft and participated in the remyelination process of the regenerated nerves. These outcomes favored functional recovery, as evidenced by improved hind limbs movement and enhanced electrophysiological properties. Thus, our study not only advanced the development of multifunctional hydrogels but also provided insights into comprehensive approaches for SCI repair. Graphical abstract Image 1 Highlights • FC/FI hydrogel exhibited excellent injectable, self-healing properties, and performed well in releasing curcumin (Cur). • FC/FI-Cur hydrogel promoted neurite outgrowth and myelination in vitro. • FC/FI-Cur modulated local inflammatory reaction and recruited Schwann cells to repair spinal cord injury. |
| Databáze: | OpenAIRE |
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