Osteoprotective Effects of Estrogen in the Maxillary Bone Depend on ERα

Autor: Tarcília Aparecida Silva, Soraia Macari, L. Ajay Sharma, David R. Grattan, Amanda Wyatt, George J. Dias, Gustavo Pompermaier Garlet, Penelope J. Knowles, Raphael E. Szawka
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Tooth Movement Techniques
Ovariectomy
Interleukin-1beta
Osteoporosis
Alveolar Bone Loss
Osteoclasts
Estrogen receptor
Apoptosis
Bone Marrow Cells
Polymerase Chain Reaction
Bone remodeling
Mice
03 medical and health sciences
0302 clinical medicine
Internal medicine
Bone cell
Maxilla
medicine
Animals
General Dentistry
Dental alveolus
Extracellular Matrix Proteins
Osteoblasts
Tumor Necrosis Factor-alpha
Chemistry
Estrogen Receptor alpha
Spectrometry
X-Ray Emission
Cell Differentiation
X-Ray Microtomography
030206 dentistry
Periodontium
Alkaline Phosphatase
Interleukin-33
medicine.disease
Mice
Inbred C57BL
Phenotype
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Calcium
Female
MAXILA
Bone Remodeling
Bone marrow
Estrogen receptor alpha
Signal Transduction
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1544-0591
0022-0345
DOI: 10.1177/0022034516633154
Popis: Estrogen deficiency results in disruption of maxillary alveolar bone microarchitecture. Most of the actions of estrogen in long bones occur via estrogen receptor α (ERα). However, the function of ERα in the maxillary bone has not been defined. We aimed to investigate the role and underlying mechanisms of ERα in the physiological and mechanically induced alveolar bone remodeling in female and male mice. Wild-type (WT) and ERα−/− (ERKOα) mice were subjected to mechanically stimulated bone remodeling by inducing orthodontic tooth movement (OTM). The maxillary bone was analyzed using histomorphometric analysis, micro–computed tomography, quantitative polymerase chain reaction, and energy-dispersive spectroscopy. Bone marrow cells (BMCs) from WT and ERKOα mice were tested for their capacity to differentiate into osteoblasts and osteoclasts. Both male and female ERKOα mice exhibited marked reduction of alveolar bone mass and increased OTM. This response was associated with an increased number of osteoclasts and reduced number of apoptotic cells and osteoblasts in the periodontium and alveolar bone. Consistently, ERKOα mice exhibited lower levels of calcium in bone and increased expression of IL-33 (interleukin-33), TNF-α (tumor necrosis factor α), and IL-1β (interleukin-1β) and decreased expression of dentin matrix acidic phosphoprotein and alkaline phosphatase in periodontal tissues. Moreover, the differentiation of osteoclasts and osteoblasts in vitro was significantly higher in BMCs obtained from ERKOα. ERα is required to maintain the microarchitecture of maxillary alveolar bone. This process is linked to bone cell differentiation and apoptosis, as well as local production of inflammatory molecules such as IL-33, TNF-α, and IL-1β.
Databáze: OpenAIRE
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