The EBV-Encoded Oncoprotein, LMP1, Recruits and Transforms Fibroblasts via an ERK-MAPK-Dependent Mechanism
| Autor: | Lawrence S. Young, Abigail Rapley, Alexandra M Davis, Mhairi A. Morris, Christopher W. Dawson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) tumour stroma Disease Biology Article Virus fibroblast 03 medical and health sciences 0302 clinical medicine EBV medicine otorhinolaryngologic diseases Immunology and Allergy Fibroblast Molecular Biology LMP1 General Immunology and Microbiology medicine.disease Phenotype In vitro myofibroblast stomatognathic diseases 030104 developmental biology Infectious Diseases medicine.anatomical_structure Nasopharyngeal carcinoma 030220 oncology & carcinogenesis Cancer research Cancer-Associated Fibroblasts Medicine NPC tumour microenvironment Myofibroblast cancer-associated fibroblasts ERK-MAPK |
| Zdroj: | Pathogens Volume 10 Issue 8 Pathogens, Vol 10, Iss 982, p 982 (2021) |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens10080982 |
| Popis: | open access article Latent membrane protein 1 (LMP1), the major oncoprotein encoded by Epstein–Barr virus (EBV), is expressed at widely variable levels in undifferentiated nasopharyngeal carcinoma (NPC) biopsies, fueling intense debate in the field as to the importance of this oncogenic protein in disease pathogenesis. LMP1-positive NPCs are reportedly more aggressive, and in a similar vein, the presence of cancer-associated fibroblasts (CAFs) surrounding “nests” of tumour cells in NPC serve as indicators of poor prognosis. However, there is currently no evidence linking LMP1 expression and the presence of CAFs in NPC. In this study, we demonstrate the ability of LMP1 to recruit fibroblasts in vitro in an ERK-MAPK-dependent mechanism, along with enhanced viability, invasiveness and transformation to a myofibroblast-like phenotype. Taken together, these findings support a putative role for LMP1 in recruiting CAFs to the tumour microenvironment in NPC, ultimately contributing to metastatic disease. |
| Databáze: | OpenAIRE |
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