Reflectometric interference spectroscopy combined with MALDI-TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives
| Autor: | Alex M. Garvin, Günter Gauglitz, Michael Steinwand, Martin Mehlmann |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Chromatography
medicine.drug_class Chemistry Analytical chemistry Biosensing Techniques Glycopeptide antibiotic Mass spectrometry MALDI-TOF Mass Spectrometry Biochemistry Sensitivity and Specificity Analytical Chemistry Anti-Bacterial Agents Matrix-assisted laser desorption/ionization Reflectometric interference spectroscopy Spectrophotometry Vancomycin Desorption Ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine Particle Size Peptides Label free |
| Zdroj: | Analytical and bioanalytical chemistry. 382(8) |
| ISSN: | 1618-2642 |
| Popis: | This paper describes the combination of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with label free bio-interaction analysis based on reflectometric interference spectroscopy (RIfS). The potential of this concerted approach is demonstrated by measuring the binding properties of different vancomycin-type glycopeptide antibiotic mixtures. Although RIfS is sensitive and does not require use of a label, it cannot determine which components of a mixture have bound to the surface after incubation. Fortunately, each bound species has a unique mass that can, afterwards, be determined by mass spectrometry. Thus, the screening capability of RIfS is combined with the identification capability of mass spectrometry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink