Characterization of macaque 3β-hydroxy-5-ene steroid dehydrogenase/Δ5-Δ4 isomerase: structure and expression in steroidogenic and peripheral tissues in primate
| Autor: | Nathalie Breton, Fernand Labrie, Hui-Fen Zhao, Jacques Simard, Laura M. Penman, Kenneth G. Low, Michael H. Melner |
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| Rok vydání: | 1991 |
| Předmět: |
Male
endocrine system Enzyme complex Molecular Sequence Data Steroid Isomerases Biology Biochemistry Macaque Endocrinology Species Specificity Multienzyme Complexes Sequence Homology Nucleic Acid Complementary DNA biology.animal Adrenal Glands Gene expression Animals Humans Amino Acid Sequence RNA Messenger Gonads Molecular Biology Peptide sequence Messenger RNA Base Sequence Progesterone Reductase cDNA library Nucleic acid sequence Membrane Proteins DNA Macaca mulatta Molecular biology Genes Organ Specificity Enzyme Induction Cattle Female |
| Zdroj: | Molecular and Cellular Endocrinology. 75:101-110 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/0303-7207(91)90224-g |
| Popis: | The conversion of 3 beta-hydroxy-5-ene steroids by the enzyme complex 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) is an obligatory step in the biosynthesis of all classes of hormonal steroids in classical steroidogenic as well as in peripheral tissues. To develop a model more closely related to the human, we have isolated and characterized cDNA clones encoding macaque 3 beta-HSD by screening a rhesus monkey ovary lambda gt11 cDNA library using a human 3 beta-HSD cDNA probe. Nucleotide sequence of 1629 bp from overlapping cDNA clones predicts a protein of 372 amino acids with a calculated molecular mass of 41,874 (excluding the first Met). The deduced amino acid sequence of macaque 3 beta-HSD displays 79.4% and 93.9% similarity with that of bovine and human 3 beta-HSD, respectively. RNA blot analysis performed under high stringency conditions of macaque poly(A)+ RNA samples using full-length 32P-labeled macaque 3 beta-HSD cDNA revealed the presence of an approximately 1.7 kb mRNA species in classical steroidogenic tissues, namely the ovary, testis and adrenal glands as well as in several peripheral tissues including the liver, kidney and epididymis. Computer analysis of the deduced macaque 3 beta-HSD protein sequence predicts the presence of an NH2-terminal membrane-associated segment as well as four additional membrane-spanning segments, thus suggesting that 3 beta-HSD is an integral protein. The availability of macaque cDNA should permit detailed studies concerning the tissue-specific expression as well as the hormonal regulation of 3 beta-HSD mRNA in classical steroidogenic glands as well as in peripheral tissues which are an important site of steroidogenesis in primates. |
| Databáze: | OpenAIRE |
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