Insights on toxin genotyping, virulence, antibiogram profiling, biofilm formation and efficacy of disinfectants on biofilms of Clostridium perfringens isolated from poultry, animals and humans
| Autor: | Amir H. Abd El-fattah, Mai Saad, Karima Abdallah, Eman Farag, Rasha M. A. Gharieb, Mariam H.E. Khedr |
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| Rok vydání: | 2020 |
| Předmět: |
Genotype
Clostridium perfringens medicine.drug_class Bacterial Toxins Antibiotics Virulence Microbial Sensitivity Tests Drug resistance Biology medicine.disease_cause Applied Microbiology and Biotechnology Poultry Microbiology 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Drug Resistance Multiple Bacterial medicine Animals Humans 030304 developmental biology 0303 health sciences 030306 microbiology Toxin Biofilm General Medicine biochemical phenomena metabolism and nutrition Anti-Bacterial Agents chemistry Biofilms Sodium hypochlorite Clostridium Infections Chickens Disinfectants Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 130:819-831 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/jam.14838 |
| Popis: | AIMS This study aimed to determine the toxin genotypes, virulence determinants and antibiogram of Clostridium perfringens isolated from poultry, animals and humans. Biofilm formation and the efficacy of disinfectants on C. perfringens biofilms were studied. METHODS AND RESULTS Thirty C. perfringens isolates (20 clinical and 10 from chicken carcasses) were genotyped by PCR and all isolates were genotype A (cpa+). The overall prevalence of cpe, cpb2, netB and tpeL virulence genes was 6·7, 56·7, 56·7 and 36·7% respectively. Twenty-one isolates (70%) were multidrug-resistant, 8 (26·7%) were extensive drug-resistant and one isolate (3·3%) was pan drug-resistant. The average multiple antibiotic resistance index was 0·7. Biofilms were produced by 63·3% of C. perfringens isolates and categorized as weak (36·7%), moderate (16·7%) and strong (10%). Sodium hypochlorite caused significant reduction in C. perfringens biofilms (P |
| Databáze: | OpenAIRE |
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