Effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rats
| Autor: | Yi Yao, Huan-Qin Cui, Wei-Yan Cai, You-Yi Gu, Ai-Min Li |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Medicine(all)
Creatinine Kidney medicine.medical_specialty Renal interstitial fibrosis Protection business.industry medicine.medical_treatment H&E stain Urology Connective tissue General Medicine Venous blood CTGF chemistry.chemical_compound medicine.anatomical_structure Diammonium glycyrrhizinate chemistry medicine Renal Interstitial Tissue Alprostadil business Saline |
| Zdroj: | Asian Pacific journal of tropical medicine. 7(11) |
| ISSN: | 2352-4146 |
| Popis: | ObjectiveTo observe effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rats.MethodsA total of 75 SD rats were randomly divided into A, B, C, D, E groups with 15 in each group. Rats in group A served as the control group received just only but tissue separation without modeling operation, while model of unilateral ureteral obstruction (UUO) was established in B, C, D, E groups. Rats in A, B group were given saline lavage placebo treatment, while rats in C, D, E groups were given diammonium glycyrrhizinate and alprostadil injection. Five rats were sacrificed 1, 2, 3 weeks after modeling, serum creatinine level of femoral venous blood was determined. Transforming growth factor - β1 (TGF - β1) and concentration of connective tissue growth factor (CTGF) were also detected by using ELISA. Line renal interstitial tissue was taken after HE staining, renal interstitial TGF - β1 and CTGF expression were detected by using immunohistochemical method.ResultsSerum creatinine levels of B, C, D, E group at different time points in were significantly higher than that of group A (P0.05); while serum and kidney tissue TGF - β1, concentration of CREA, expression of rats in B, C, D, E groups showed a gradual increasing trend over time. TGF - β1 and CREF of Group B in serum and kidney tissues at each time point were significantly higher than that of the other groups (P |
| Databáze: | OpenAIRE |
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