Interleukin-4 regulates connective tissue growth factor expression in human lung fibroblasts
| Autor: | Ronald H. Goldstein, Ping-Ping Kuang, Hanqiao Liu, David C. Rishikof, Dennis A. Ricupero |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_treatment
MAP Kinase Kinase Kinase 1 Connective tissue SMAD Protein Serine-Threonine Kinases Biology Biochemistry Collagen Type I Immediate-Early Proteins Phosphatidylinositol 3-Kinases Transforming Growth Factor beta Fibrosis medicine Humans Insulin RNA Messenger Northern blot Enzyme Inhibitors Promoter Regions Genetic Lung Molecular Biology Phosphoinositide-3 Kinase Inhibitors Dose-Response Relationship Drug integumentary system Growth factor Connective Tissue Growth Factor Cell Biology Fibroblasts medicine.disease Molecular biology Fibronectins CTGF Fibronectin medicine.anatomical_structure Gene Expression Regulation Dactinomycin biology.protein Intercellular Signaling Peptides and Proteins Interleukin-4 Transforming growth factor |
| Zdroj: | Journal of Cellular Biochemistry. 85:496-504 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.10144 |
| Popis: | Transforming growth factor-β (TGF-β) and interleukin-4 (IL-4) have fibrogenic properties and induce extracellular matrix production in a variety of lung diseases. Connective tissue growth factor (CTGF) is a matrix signaling molecule stimulated by TGF-β that in part mediates α1(I) collagen mRNA expression. In these studies, the regulation of CTGF expression by IL-4 in human lung fibroblasts was examined. Following 6 h of stimulation with IL-4, basal CTGF mRNA levels were unchanged as assessed by Northern blot analysis. However, IL-4 attenuated the TGF-β-stimulated induction of CTGF mRNA expression by 50%. This effect was selective because IL-4 did not affect fibronectin or α1(I) collagen mRNA expression induced by TGF-β. Experiments employing the transcriptional inhibitor actinomycin D suggest that IL-4 did not affect the stability of the CTGF mRNA. Transient transfection assays with 3TP-Lux, a luciferase gene controlled by a TGF-β inducible promoter, and with a CTGF promoter construct indicate that IL-4 interfered with the TGF-β-induced transcriptional activation of the CTGF gene. J. Cell. Biochem. 85: 496–504, 2002. © 2002 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text