Anticancer activity and metabolite profiling data of Penicillium janthinellum KTMT5
| Autor: | Kudzanai Ian Tapfuma, Lukhanyo Mekuto, Raeesa Hussan, Nkemdinma Uche-Okereafor, Jody Koopman, Tendani Edith Sebola, Maya M. Makatini, Vuyo Mavumengwana |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Penicillium janthinellum
Mine tailings 0303 health sciences Multidisciplinary Natural product biology biology.organism_classification Tandem mass spectrometry DNA sequencing LC-QTOF-MS/MS 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Anticancer chemistry Biochemistry Metabolite profiling Cell culture Biochemistry Genetics and Molecular Biology Penicillium IC50 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Data in Brief |
| ISSN: | 2352-3409 |
| Popis: | Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of Penicillium janthinellum KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of P. janthinellum KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. P. janthinellum KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC50 value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of P. janthinellum KTMT5 and molecular networking showed the relationships among these metabolites. |
| Databáze: | OpenAIRE |
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