Bacterial biofilm in adenoids of children with chronic otitis media. Part II: a case-control study of nasopharyngeal microbiota, virulence, and resistance of biofilms in adenoids
| Autor: | Natacha Tessier, Emmanuelle Cambau, Benjamin Verillaud, Pierre Vironneau, Emmanuelle Varon, Philippe Herman, Gerda E. M. Lamers, Romain Kania, Huong Dang, Eric Vicaut, Thierry Van Den Abbeele, Hervé Jacquier, Béatrice Berçot, Philippe Bidet |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Firmicutes Streptococcus pyogenes Virulence Microbial Sensitivity Tests medicine.disease_cause Adenoid Actinobacteria Microbiology Haemophilus influenzae 03 medical and health sciences 0302 clinical medicine Nasopharynx Streptococcus pneumoniae Drug Resistance Bacterial medicine Humans 030223 otorhinolaryngology Child Analysis of Variance biology Bacteria Biofilm General Medicine biology.organism_classification Otitis Media medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Biofilms Case-Control Studies Adenoids Chronic Disease |
| Zdroj: | Acta oto-laryngologica. 140(3) |
| ISSN: | 1651-2251 |
| Popis: | Background: We previously described that adenoid tissue in children with chronic otitis media (COM) contained more mucosal biofilms than adenoid tissue removed for hypertrophy.Aims/objectives: The aim of the second part was to characterize nasopharyngeal microbiota and explore virulence of the most common middle ear pathogens.Material and methods: Bacteriological analysis was performed following a culture-based approach on the samples recovered from 30 patients of COM group (15 biofilm-positive and 15 biofilm-negative) and from 30 patients of a control group (15 biofilm-positive and 15 biofilm-negative). Virulence factors of Streptococcus pneumoniae, Streptococcus pyogenes, and Haemophilus influenzae were investigated.Results: The most frequent species were Firmicutes followed by Proteobacteria and Actinobacteria. The presence of biofilm was statistically associated with an increase of the number of bacterial species and Firmicutes phylum regardless of the condition (case/control). No virulence factors associated with invasive isolates were found for the most common middle ear pathogens.Conclusions and significance: This case-control study demonstrated that the presence of COM plus biofilm was associated with a given microbiota which contained more Firmicutes. Our study allows a better understanding of physiopathological mechanisms involved in chronic otitis media and paves the way for further investigations. |
| Databáze: | OpenAIRE |
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