V-ATPase subunit a is required for survival and midgut development of Locusta migratoria
| Autor: | X.-J. Liu, Jianzhen Zhang, Kun Yan Zhu, X.-Y. Liang, X.-K. Shi, H. Merzendorfer, J. Guo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adenosine Triphosphatases
Nymph Vacuolar Proton-Translocating ATPases biology Brush border ATPase Protein subunit Locusta migratoria Midgut Cell biology Cytoplasm RNA interference Insect Science Complementary DNA Genetics biology.protein V-ATPase Animals Insect Proteins RNA Interference Molecular Biology RNA Double-Stranded |
| Zdroj: | Insect molecular biologyReferences. 31(1) |
| ISSN: | 1365-2583 |
| Popis: | The vacuolar-type H+ -ATPase (V-ATPase) is an ATP-dependent proton pump, which regulates various cellular processes. To date, most functional studies on V-ATPases of insects have focused on subunits of the V1 complex, and there is little information on the VO genes. In this study, two cDNA sequences of LmV-ATPase a were identified in Locusta migratoria. RT-qPCR analysis revealed that LmV-ATPase a1 and LmV-ATPase a2 are differentially expressed in various tissues and developmental stages. Injection of dsRNA for the common region of LmV-ATPase a1 and LmV-ATPase a2 into third-instar nymphs resulted in a significant suppression of LmV-ATPase a. The injected nymphs ceased feeding, lost body weight, and finally died at a mortality of 98.6%. Furthermore, aberrations of midgut epithelial cells, the accumulation of electron-lucent vesicles in the cytoplasm, and a partially damaged brush border were observed in dsLmV-ATPase a injected nymphs using transmission electron microscopy. Especially, the mRNA level of wingles, and notch genes were dramatically down-regulated in the dsLmV-ATPase a-injected nymphs. Taken together, our results suggest that LmV-ATPase a is required for survival and midgut development of L. migratoria. Hence, this gene could be a good target for RNAi-based control against locusts. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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