A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system
| Autor: | Maryam Saleh, Abbas Jamali, Peyvand Biglari, Asghar Abdoli, Mohammad Hasan Samiee Aref, Yahya Mohammadzadeh, Narges Rasouli, Masoumeh Tavassoti Kheiri, M Tabatabaeian, Nasim Sadat Seyed Tabib |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Virosomes viruses Hemagglutinin (influenza) Bioengineering Gene delivery Transfection Applied Microbiology and Biotechnology 03 medical and health sciences Vesicular Stomatitis Viral Proteins 0302 clinical medicine chemistry.chemical_classification biology fungi HEK 293 cells Gene Transfer Techniques virus diseases General Medicine biology.organism_classification Virology Virosome 030104 developmental biology chemistry Vesicular stomatitis virus 030220 oncology & carcinogenesis embryonic structures biology.protein Glycoprotein Biotechnology |
| Zdroj: | Biotechnology letters. 38(8) |
| ISSN: | 1573-6776 |
| Popis: | To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome. A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis. The chimeric virosome was significantly more efficient in mediating transfection for all amounts of DNA and virosomes compared to the influenza virosome. Chimeric influenza virosome, including VSV-G, is superior to the conventional influenza virosome for gene delivery. |
| Databáze: | OpenAIRE |
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