NKG2D receptor–mediated NK cell function is regulated by inhibitory Ly49 receptors
| Autor: | Subramaniam Malarkannan, Jeyarani Regunathan, Yuhong Chen, Demin Wang |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cytotoxicity
Immunologic NK Cell Lectin-Like Receptor Subfamily K Immunology Active Transport Cell Nucleus Down-Regulation chemical and pharmacologic phenomena Immune receptor Biology Ligands Major histocompatibility complex Biochemistry Article Natural killer cell Minor Histocompatibility Antigens Mice Immune system medicine Animals Antigens Ly Humans Lectins C-Type Receptors Immunologic Histocompatibility Antigen H-2D Receptor Cells Cultured Cell Nucleus H-2 Antigens NF-kappa B Antibodies Monoclonal Cell Biology Hematology Molecular biology Cell biology Killer Cells Natural medicine.anatomical_structure biology.protein Receptors Natural Killer Cell Cytokine secretion Signal transduction NK Cell Lectin-Like Receptor Subfamily A Receptors NK Cell Lectin-Like Signal Transduction |
| Zdroj: | Blood. 105:233-240 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2004-03-1075 |
| Popis: | Interaction of the activating ligand H60 with NKG2D receptor constitutes a major stimulatory pathway for natural killer (NK) cells. The influence of inhibitory Ly49 receptors on NKG2D-mediated activation is not clearly understood. Here we show that the magnitude of NKG2D-mediated cytotoxicity is directly proportional to both the levels of H60 and the nature of major histocompatibility complex (MHC) class I molecules expressed on the target cells. The expression levels of H60 on the target cells determined the extent to which the inhibition by Ly49C/I receptors can be overridden. In contrast, even a higher expression of H60 molecule on the target cells failed to overcome the inhibition mediated by Ly49A/G receptors. Also, the level of interferon-γ (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) generated by NK cells through anti-NKG2D monoclonal antibody (mAb)-mediated activation is significantly reduced by the presence of immobilized anti-Ly49A/G mAbs. Thus, NKG2D-mediated cytotoxicity and cytokine secretion results from the fine balance between activating and inhibitory receptors, thereby defining the NK cell-mediated immune responses. (Blood. 2005;105:233-240) |
| Databáze: | OpenAIRE |
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