Knockdown of HSDL2 inhibits lung adenocarcinoma progression via down-regulating AKT2 expression

Autor: Zheng-Dao Mao, Xiao-Wei Yin, Jianan Huang, Yanhua Huang, Qian Zhang, Yun Sun, Qi Cao, Yujia Shi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Lung Neoplasms
AKT2
Apoptosis
Biochemistry
0302 clinical medicine
Cell Movement
Gene expression
RNA
Small Interfering
Pneumonectomy
Lung
Research Articles
Cancer
Gene knockdown
Chemistry
Cell cycle
Middle Aged
Immunohistochemistry
Up-Regulation
Gene Expression Regulation
Neoplastic
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Disease Progression
Adenocarcinoma
Female
gene chip
Biophysics
Down-Regulation
Adenocarcinoma of Lung
03 medical and health sciences
hydroxysteroid dehydrogenase-like 2
Cell Line
Tumor
Proto-Oncogene Proteins
medicine
Humans
Neoplasm Invasiveness
Molecular Biology
Protein kinase B
non-small cell lung cancer
Cell Proliferation
Oncogene
Gene Expression & Regulation
Cell growth
Hydroxysteroid Dehydrogenases
Cell Biology
medicine.disease
lung adenocarcinoma
Signaling
030104 developmental biology
Cancer research
prognosis
Proto-Oncogene Proteins c-akt
Zdroj: Bioscience Reports
ISSN: 1573-4935
0144-8463
Popis: The aims of the present study are to investigate the role of hydroxysteroid dehydrogenase-like 2 (HSDL2) in the progression of lung adenocarcinoma and illuminate the underlying molecular mechanisms. ShRNA targeting HSDL2 gene (siHSDL2) was utilized to knockdown (KD) HSDL2 expression. In vitro and in vivo experiments were carried out to investigate the effect of siHSDL2 on the progression of lung adenocarcinoma. Microarray hybridization and gene expression analysis were used to investigate effect of siHSDL2 on mRNA expression profile in lung cancer cell line H1299. Our data demonstrated that HSDL2 was up-regulated in lung adenocarcinoma tissue samples (P
Databáze: OpenAIRE
Externí odkaz: