A study of the pyrogenic actions of interleukin-1α and interleukin-1β: interactions with a steroidal and a non-steroidal anti-inflammatory agent
| Autor: | J. Davidson, A.S. Milton, D. Rotondo |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Ketoprofen medicine.medical_treatment Anti-Inflammatory Agents Alpha (ethology) Pharmacology Dexamethasone Body Temperature medicine Animals Drug Interactions Beta (finance) biology Pyrogens business.industry Anti-Inflammatory Agents Non-Steroidal Interleukin Biological activity Recombinant Proteins Cytokine Enzyme inhibitor biology.protein Rabbits business Interleukin-1 Research Article medicine.drug |
| Zdroj: | British Journal of Pharmacology. 100:542-546 |
| ISSN: | 0007-1188 |
| DOI: | 10.1111/j.1476-5381.1990.tb15843.x |
| Popis: | 1. The pyrogenic effects of intravenously administered human recombinant interleukin-1 alpha (IL-1 alpha) and IL-1 beta were studied in the rabbit. 2. Both cytokines produced dose-related increases in body temperature. At all doses studied (100-5000 u kg-1) both cytokines elicited a monophasic increase in body temperature, beginning 15 min and reaching a maximum 45 min after administration. 3. A comparison of thermal response index (TRI2, the magnitude of febrile responses over 2 h obtained by integrating the change in temperature in degrees C against time in hours) values indicated that IL-1 beta (500 u kg-1, TRI2 = 0.69 +/- 0.04, n = 4) was approximately 5 fold more potent than IL-1 alpha (2500 u kg-1, TRI2 = 0.73 +/- 0.07, n = 4, all values are means +/- s.e.means) in elevating body temperature. delta Tmax values for the above doses of IL-1 beta and IL-1 alpha were 0.60 +/- 0.06 and 0.61 +/- 0.03 respectively. When IL-1 alpha and IL-1 beta were heated for 30 min at 60 degrees C prior to administration no biological activity was observed. 4. A cyclo-oxygenase inhibitor, ketoprofen (3 mg kg-1) administered 15 min before either cytokine completely abolished the fever induced by both IL-1 alpha (2500 u kg-1) and IL-1 beta (500 u kg-1). 5. Intravenous administration of the steroidal anti-inflammatory agent dexamethasone (3 mg kg-1) 1 h before either cytokine attenuated the fever induced by IL-1 alpha (2500 u kg-1) and IL-1 beta (500 u kg-1). 6. The effects of ketoprofen and dexamethasone on IL-I pyrogenicity indicate that prostanoids are almost certainly involved in the responses. The different potencies of IL-l alpha and IL-1 beta may be related to their relative ability to stimulate prostanoid biosynthesis. |
| Databáze: | OpenAIRE |
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