Engineering a sustained release vaccine with a pathogen-mimicking manner for robust and durable immune responses
| Autor: | Rong Liu, Shuting Bai, Zhirong Zhang, Yunting Zhang, Tao Gong, Xianjin Luo, Yuandong Zhang, Y.P. Hou, Xiaoyu Hong, Xun Sun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cellular immunity
medicine.medical_treatment Pharmaceutical Science 02 engineering and technology Mice 03 medical and health sciences Immune system Adjuvants Immunologic Antigen medicine Animals Vector (molecular biology) Receptor 030304 developmental biology Immunity Cellular Vaccines 0303 health sciences Chemistry 021001 nanoscience & nanotechnology Immunity Humoral Mice Inbred C57BL Vaccination Delayed-Action Preparations Immunology Cytokine secretion 0210 nano-technology Adjuvant |
| Zdroj: | Journal of Controlled Release. 333:162-175 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2021.03.037 |
| Popis: | Sustained release vaccine carriers can facilitate an increased interaction time between the antigen and immune system to strengthen immune responses, but their promotion on adaptive immune responses, especially cellular immunity, are still unfavorable. Herein, we report a sustained antigen delivery vector, which carries abundant antigens, a nucleic acid adjuvant and pathogen-associated molecular patterns to simulate a natural pathogen to reinforce immune responses. Specifically, murine colorectal cancer cells MC38 lysate and Toll-like receptor 9 agonist CpG are loaded into yeast derived β-glucan particles (GPs). After vaccination, these particles can form a vaccine depot that continuously release the antigen similar to the traditional aluminum hydroxide gel, but recruit more immune cells and induce more cytokine secretion at the injection site. Stronger antibody responses, Th1 and Th17 biased cellular immunity and immune memory are achieved compared with aluminum hydroxide gel. More importantly, treatment with these particles significantly suppress tumor growth in a therapeutic tumor model. This work shed light on the efficacy of combining sustained antigen release with pathogen-mimicking manner in vaccine design. |
| Databáze: | OpenAIRE |
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