Designing an immunoinformatic vaccine for peri-implantitis using a structural biology approach
| Autor: | Amit Porwal, Mohammed E. Sayed, Shankargouda Patil, Khaled M. Alzahrani, Maryam H. Mugri, Pradeep Kumar Yadalam, Nasser M. Alahmari, Ali Robaian, Santhiya Rengaraj, Hosam Ali Baeshen |
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| Rok vydání: | 2022 |
| Předmět: |
CXCR4
education.field_of_study Receptor complex Antigenicity QH301-705.5 Population Biology Acquired immune system Major histocompatibility complex biology.organism_classification Epitope Vaccination Immunoinformatics approach Multi-epitope vaccine Immunology biology.protein Original Article Peri-implantitis Biology (General) General Agricultural and Biological Sciences education Porphyromonas gingivalis |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 29, Iss 1, Pp 622-629 (2022) Saudi Journal of Biological Sciences |
| ISSN: | 1319-562X |
| Popis: | Objectives Peri-implantitis is a destructive inflammatory process that affects the soft and hard tissues around dental implants. porphyromonas gingivalis, an anaerobic gram-negative bacterium, appears to be the main culprit. Since there is no efficient and specific vaccine to treat peri-implantitis, the goal of our research has been to develop a multi-epitope vaccination utilizing an immunoinformatics approach that targeted P. gingivalis type I fim A. Materials and methods P. gingivalis peptides 6JKZ and 6KMF are suitable for vaccine development. B- and T-cell epitopes from 6KMF and 6JKZ were detected and evaluated based on critical factors to produce a multi-epitope vaccine construct. It was assessed based on allergenicity, antigenicity, stability. The vaccine's dual major histocompatibility complex (MHC-I and MHC-II) binding epitopes allowed it to reach a larger population. P. gingivalis fimbriae induce immune subversion through TLR -CXCR4 receptor complex pathway. The ClusPro 2.0 server was used to do the molecular docking using TLR2 - CXCR4 and vaccine epitopes as receptor and ligand respectively. Results The designed vaccine was non-allergenic and had a high antigenicity, solubility, and stability. The 3D structure of the vaccine revealed strong interaction with CXCR4(TLR2) using molecular docking. The vaccine-CXCR4 interface was more consistent, possibly because the vaccination has a higher affinity for the CXCR4-TLR2 complex. Conclusion This study details the vaccine's distinct and sustained interaction with the CXCR4(TLR2) immunological receptor and its consistent and effective utterance in the bacterial system. As a result, our vaccine formulation will evoke a significant memory response and induce an adaptive immune response against P. gingivalis. |
| Databáze: | OpenAIRE |
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