Penicillium stoloniferum Virus: Altered Replication in Ultraviolet-derived Mutants
| Autor: | C. W. Hesseltine, P. E. Still, R. W. Detroy |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Ultraviolet Rays
Mutant Color Biology Virus Replication Virus Plant Viruses Microbiology Fungal Proteins Virology Ultraviolet light RNA Viruses Polyacrylamide gel electrophoresis Mycelium Strain (chemistry) Penicillium Wild type DNA Spores Fungal Molecular biology Radiation Effects Phenotype Viral replication Mutation RNA RNA Viral Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of General Virology. 27:275-281 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-27-3-275 |
| Popis: | SUMMARY Phenotypic mutants of the wild type of Penicilliu/ll stoloniferul11 NRRL 5267 were obtained from conidia exposed to ultraviolet light for 60 min (10 survival). Virus content of the wild type and of nine phenotypic mutants was determined by polyacrylamide gel electrophoresis. Four mutants had no detectable Penicilliul11 stoloniferul11 virus F (PsV-F), whereas the other five had levels of PsV-F in the mycelium similar to the wild-type strain. All nine mutants and the wild type had comparable levels of Penicilliul11 stoloniferul11 virus S (PsV-S). Maximum virus levels occurred after 9 days of submerged culture in a 2 yeast extract-I5 sucrose medium. Virus replication in the fungal host continued after protein, RNA and DNA synthesis levelled off. Virus levels ranged from 85 to 150 E2GO units (extinction units at 260 nm in I cm cell) per 4'7 to 5'3 g dry weight of mycelium for the mutant strains compared to 106 E260 units per 4'2 g dry weight of the wild-type strain. |
| Databáze: | OpenAIRE |
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